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- 2016
高脂饮食影响雌性大鼠对糖精溶液的喜好并改变多巴胺及阿片相关基因的表达
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Abstract:
摘要：目的 ?探讨高脂饮食对雌性大鼠糖精溶液喜好的影响及其可能机制。方法? ?成年雌性大鼠分成普通饮食组(CHOW组，13.0%热卡来自脂肪)及高脂饮食组(HF组，50.5%热卡来自脂肪)。喂养9周后，称量体质量，检测血浆中的瘦素水平；测量大鼠在双瓶选择实验中对不同浓度糖精溶液的摄入量及喜好率；运用实时定量PCR检测奖赏相关核团及下丘脑中多巴胺及阿片相关基因的表达。结果? ?与CHOW组相比，HF组有较高的体质量及血浆瘦素水平；HF组对低浓度(0.001mol/L)糖精溶液的摄入量及喜好率较低，而对高浓度(0.02、0.04mol/L)糖精溶液的摄入量及喜好率则较高；在奖赏相关核团中，HF组多巴胺再摄取转运体(dopamine reuptake transporter, DAT)的mRNA表达显著增加，多巴胺D1受体、多巴胺D2受体及DARPP-32蛋白的mRNA表达较低，以及μ阿片受体(μ-opioid receptor, MOR)的mRNA表达降低。结论?? 高脂饮食影响雌性大鼠对糖精溶液的喜好率，该作用可能部分是由奖赏核团中多巴胺及阿片系统的变化导致的“低奖赏”状态引起的。
ABSTRACT: Objective? ?To determine whether high-fat (HF) diet affects preference for saccharin solutions in female rats and the possible underlying mechanisms. Methods?? Adult female Sprague-Dawley rats were divided into 2 groups: CHOW group was fed with a normal chow diet (13.0% calories from fat) and HF group with an HF diet (50.5% calories from fat). At the end of the 9th week, we measured body weight gain and plasma leptin levels, saccharin intake and preference using a two-bottle preference test, and dopamine and opioid related gene expression in reward-related nuclei and hypothalamus using real-time PCR. Results? ?Compared with CHOW group, HF group had greater body weight gain and higher plasma leptin levels. The consumption and preference ratio for 0.001mol/L saccharin were lower in the HF rats, while the consumption and preference ratios for 0.02 and 0.04mol/L saccharin were higher in these rats. HF group had higher dopamine reuptake transporter (DAT) mRNA expression, lower dopamine receptor D1, dopamine receptor D2 and DARPP-32 dopamine- and cAMP-regulated phosphoprotein mRNA expression, and lower μ-opioid receptor (MOR) mRNA expression in reward-related nuclei. Conclusion? HF diet affects preference for saccharin solution in female rats, and it may be partially due to the state of reward hypofunction induced by alterations of dopamine and opioid system in reward-related nuclei
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