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- 2018
大鼠关节软骨发育不同阶段12种硒蛋白的表达
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Abstract:
摘要：目的 获得大鼠骨软骨发育不同阶段关节软骨细胞硒蛋白表达谱。方法 选择出生后7个不同发育时间点各10只近交系DA大鼠的关节软骨细胞，RT-PCR方法检测12种硒蛋白的表达差异，RT-qPCR定量。免疫组化验证表达结果。结果 所选定的骨软骨发育不同阶段关节软骨细胞中Gpx1、Gpx2、Gpx3、Gpx4、Dio2、SelS呈现不同程度的差异表达，且RT-qPCR显示Gpx1和Dio2随着软骨生成而表达升高(P<0.05)，Gpx2和Gpx4稳定表达。免疫组化结果证实GPX1在性成熟大鼠股骨骺板特异高表达。结论 6种硒蛋白可能在大鼠骨软骨发育的增殖与分化过程中发挥着重要的作用。
ABSTRACT: Objective To observe the expressions of selenoproteins in rat cartilage at different developmental stages. Methods The femoral head cartilage of inbred DA rats at 7 different developmental stages (each group contained 10 rats) was collected to extract total RNA with Trizol method. The expressions of 12 kinds of selenoproteins in the femoral head cartilage were determined by the semiquantitative RT-PCR. The abundance of selenoprotein expression was quantitated with RT-qPCR assay. The results were comfirmed by immunohistochemistry. Results At different skeletal developmental stages, Gpx1, Gpx2, Gpx3, Gpx4 and Dio2 presented significantly different expressions in cartilage chondrocytes. RT-qPCR assay showed that Gpx1 and Dio2 were significantly up-regulated (P<0.05) while Gpx2 and Gpx4 had steady expressions. Immunohistochemistry assay result confirmed that GPX1 was expressed in a high percentage of epiphyseal plate in sexually mature rats. Conclusion There are significant differences in the gene expressions of selenoproteins in rat development, suggesting that selenoproteins may play an important role in proliferation and differentiation processes of rat bone-cartilage development
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