Metrigel-VEGF-SW982细胞支架复合物的构建及在滑膜肉瘤小鼠模型上的应用
Establishment of Metrigel-VEGF-SW982 complexes and application of the complexes in synovial sarcoma model in mice

摘要：目的 建立Metrigel-VEGF-SW982细胞支架复合物并利用复合物制作移植瘤动物模型，为其他软组织肿瘤动物模型的建立提供思路。方法 培养扩增SW982细胞，消化收集细胞，用Metrigel对SW982细胞进行重悬，同时加入VEGF，应用Transwell制作Metrigel-VEGF-SW982细胞支架复合物，并进行培养，HE染色观察细胞支架复合物形态结构。选取10只4周龄雌性SCID小鼠，随机分成支架组和对照组。支架组小鼠腋下移植细胞支架复合物，对照组注射移植细胞培养基悬液。8周后，比较两组造模成功率，取材HE染色观察肿瘤的组织形态特点。结果 利用Metrigel对SW982细胞进行立体式培养，能够很好的模拟细胞在体的生长状态。采用细胞支架复合物构建滑膜肉瘤动物模型，成瘤率较对照组明显提高，且肿瘤体积更大，细胞密度更高，肿瘤内部有新生血管。结论 利用Metrigel-VEGF-SW982细胞支架复合物构建滑膜肉瘤动物模型，能够极大地提高模型成瘤率，为滑膜肉瘤的研究提供便利。
ABSTRACT: Objective To establish Metrigel-VEGF-SW982 complexes and use the complexes to produce animal models of synovial sarcoma so as to provide new ideas for establishing other models of soft tissue tumors. Methods After the SW982 cells were cultured and collected, they were resuspended with Metrigel, and VEGF was added. The suspension was seeded into transwell to establish the scaffold complexes of Metrigel-VEGF-SW982. The complexes were cultured overnight. Cryosections were made and HE staining was carried out to observe the cell scaffold complexes. We randomly divided 10 female SCID mice (4 week old) into scaffold group and control group. The mice in the scaffold group were transplanted with cell-scaffold complexes, and the control group with cell suspension. After 8 weeks, the success rate of modeling was compared between two groups. The mice were sacrificed and the tumors were obtained. HE staining was carried out to observe the histopathological features of tumors in both scaffold group and control group. Results The SW982 cells were cultured with Matrigel in a 3D way, which could simulate the growth condition of cells in vivo. Establishing synovial sarcoma animal model with cell scaffold complex could increase the success rate. The tumors in scaffold group had a larger volume, higher density of tumor cells and greater vascularization (P<0.05). Conclusion Establishing synovial sarcoma animal model with Metrigel-VEGF-SW982 complex can greatly improve the success rate of modeling, which can provide basis for the study of synovial sarcoma