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-  2015 

肝癌肿瘤微环境对血管内皮细胞增殖及血管生成能力的影响

DOI: doi:10.7507/1001-5515.20150111

Keywords: 肝癌细胞, 血管内皮细胞, 细胞增殖, 血管生成

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Abstract:

研究间接共培养条件下肝癌细胞培养基对血管内皮细胞增殖及血管生成能力的影响, 初步探讨肿瘤微环境下血管新生的分子机制。体外培养人脐静脉内皮细胞株EA.hy926, 与肝癌细胞株HepG2条件培养基进行共培养; 四甲基偶氮唑盐(MTT)法检测肝癌细胞条件培养基对血管内皮细胞增殖的影响; 血管管腔形成实验检测血管内皮细胞的血管生成能力。Western blot测定肝癌细胞条件培养基对血管内皮EA.hy926细胞血管内皮生长因子(VEGF)及其受体Flk-1表达的影响。结果表明, EA.hy926细胞经HepG2条件培养基处理后, 其增殖能力明显增加。接种于Matrigel胶的EA.hy926细胞经HepG2条件培养基刺激后, 形成管腔数目增加, 成血管能力明显增强; 同时, 其胞内VEGF及其受体Flk-1的表达呈时间依赖性上调。肿瘤微环境下肝癌细胞作用于血管内皮细胞, 可促进血管内皮细胞的增殖并提高其血管生成能力

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