14. Peng Jin, Wang Yong, Jiang Junyi, et al. Production of human albumin in pigs through CRISPR/Cas9-mediated knockin of human cDNA into swine albumin locus in the zygotes. Sci Rep, 2015, 5: 16705.
[2]
15. Wu Jun, Vilarino M, Suzuki K, et al. CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs. Sci Rep, 2017, 7(1): 10487.
[3]
27. Wang Xianlong, Cao Chunwei, Huang Jiaojiao, et al. One-step generation of triple gene-targeted pigs using CRISPR/Cas9 system. Sci Rep, 2016, 6: 20620.
[4]
37. Zhang Yongliang, Xi Qianyun, Ding Jinghua, et al. Production of transgenic pigs mediated by pseudotyped lentivirus and sperm. PLoS One, 2012, 7(4): e35335.
[5]
2. Niemann H, Petersen B. The production of multi-transgenic pigs: update and perspectives for xenotransplantation. Transgenic Res, 2016, 25(3): 361-374.
[6]
3. Butler J R, Ladowski J M, Martens G R, et al. Recent advances in genome editing and creation of genetically modified pigs. Int J Surg, 2015, 23(Pt B): 217-222.
[7]
4. Song Chanwoo, Lee J, Lee S Y. Genome engineering and gene expression control for bacterial strain development. Biotechnol J, 2015, 10(1): 56-68.
[8]
5. Eid A, Mahfouz M M. Genome editing: the road of CRISPR/Cas9 from bench to clinic. Exp Mol Med, 2016, 48(10): e265.
[9]
6. Ulain Q, Chung J Y, Kim Y H. Current and future delivery systems for engineered nucleases: ZFN, TALEN and RGEN. J Control Release, 2015, 205: 120-127.
[10]
7. Samanta M K, Dey A, Gayen S. CRISPR/Cas9: an advanced tool for editing plant genomes. Transgenic Res, 2016, 25(5): 561-573.
[11]
8. Lafountaine J S, Fathe K, Smyth H D. Delivery and therapeutic applications of gene editing technologies ZFNs, TALENs, and CRISPR/Cas9. Int J Pharm, 2015, 494(1): 180-194.
[12]
13. Wang Haoyi, Yang Hui, Shivalila C S, et al. One-Step Generation of mice carrying mutations in multiple genes by CRISPR/Cas-Mediated genome engineering. Cell, 2013, 153(4): 910-918.
[13]
16. Lei Shaohua, Ryu J, Wen Ke, et al. Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency. Sci Rep, 2016, 6: 25222.
[14]
17. Sato M, Koriyama M, Watanabe S, et al. Direct injection of CRISPR/Cas9-Related mRNA into cytoplasm of partheno genetically activated porcine oocytes causes frequent mosaicism for indel mutations. Int J Mol Sci, 2015, 16(8): 17838-17856.
[15]
18. Wang Xianlong, Zhou Jinwei, Cao Chunwei, et al. Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Sci Rep, 2015, 5: 13348.
[16]
19. Wu Jinqing, Mei Gui, Liu Zhiguo, et al. Improving gene targeting efficiency on pig IGF2 mediated by ZFNs and CRISPR/Cas9 by using SSA reporter system. Yi Chuan, 2015, 37(1): 55-62.
[17]
20. Tao Li, Yang Mingyao, Wang Xiaodong, et al. Efficient biallelic mutation in porcine parthenotes using a CRISPR-Cas9 system. Biochem Biophys Res Commun, 2016, 476(4): 225-229.
[18]
21. Wang Kepin, Jin Qin, Ruan Degong, et al. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing. Genome Res, 2017, 27(12): 2061-2071.
[19]
22. Hai Tang, Teng Fei, Guo Runfa, et al. One-step Generation of knockout pigs by zygote injection of CRISPR/Cas system. Cell Res, 2014, 24(3): 372-375.
[20]
23. Lotem J, Levanon D, Negreanu V, et al. Runx3 at the interface of immunity, inflammation and cancer. Biochim Biophys Acta, 2015, 1855(2): 131-143.
[21]
24. Kang J T, Ryu J, Cho B, et al. Generation of RUNX3 knockout pigs using CRISPR/Cas9-mediated gene targeting. Reproduction in Domestic Animals, 2016, 51(6): 970-978.
[22]
25. Wang Kankan, Ouyang Hongsheng, Xie Zicong, et al. Efficient Generation of myostatin mutations in pigs using the CRISPR/Cas9 system. Sci Rep, 2015, 5: 16623.
[23]
26. Yan Sen, Tu Zhuchi, Liu Zhaoming, et al. A huntingtin knockin pig model recapitulates features of selective neurodegeneration in huntington's disease. Cell, 2018, 173(4): 989-1002.
[24]
28. Yang Luhan, Gueell M, Niu Dong, et al. Genome-wide inactivation of porcine endogenous retroviruses (PERVs). Science, 2015, 350(6264): 1101-1104.
[25]
29. Niu Dong, Wei Hongjiang, Lin Lin, et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. Science, 2017, 357(6357): 1303-1307.
[26]
30. Estrada J L, Martens G, Li Ping, et al. Evaluation of human and non-human Primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes. Xenotransplantation, 2015, 22(3): 194-202.
[27]
35. Wu Zhenfang, Li Zicong, Yang Jinzeng. Transient transgene transmission to piglets by intrauterine insemination of spermatozoa incubated with DNA fragments. Mol Reprod Dev, 2008, 75(1): 26-32.
[28]
36. Lavitrano M, Giovannoni R, Cerrito M G. Methods for sperm-mediated gene transfer. Methods Mol Biol, 2013, 927: 519-529.
[29]
31. Butler J R, Paris L L, Blankenship R L, et al. Silencing porcine CMAH and GGTA1 genes significantly reduces xenogeneic consumption of human platelets by porcine livers. Transplantation, 2016, 100(3): 571-576.
[30]
32. Gao Hanchao, Zhao Chengjiang, Xiang Xi, et al. Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning. J Reprod Dev, 2017, 63(1): 17-26.
[31]
33. Petersen B, Frenzel A, Lucas-Hahn A, et al. Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotes. Xenotransplantation, 2016, 23(5): 338-346.
[32]
34. Kang J T, Cho B, Ryu J, et al. Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs. Reprod Biol Endocrinol, 2016, 14(1): 74.
[33]
38. Oddi S, Bernabò N, Di Tommaso M, et al. DNA uptake in swine sperm: effect of plasmid topology and methyl-beta-cyclodextrin-mediated cholesterol depletion. Mol Reprod Dev, 2012, 79(12): 853-860.
[34]
1. Whyte J J, Prather R S. Genetic modifications of pigs for medicine and agriculture. Mol Reprod Dev, 2011, 78(10/11): 879-891.
[35]
9. Chen Fengjiao, Wang Ying, Yuan Yilin, et al. Generation of B cell-deficient pigs by highly efficient CRISPR/Cas9-mediated gene targeting. Journal of Genetics and Genomics, 2015, 42(8): 437-444.
[36]
10. Zhou Xiaoqing, Xin Jige, Fan Nana, et al. Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cellular and Molecular Life Sciences, 2015, 72(6): 1175-1184.
[37]
11. Bi Yanzhen, Hua Zaidong, Liu Ximei, et al. Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP. Sci Rep, 2016, 6: 31729.
[38]
12. Lai Sisi, Wei Shu, Zhao Bentian, et al. Generation of knock-in pigs carrying Oct4-tdTomato reporter through CRISPR/Cas9-mediated genome engineering. PLoS One, 2016, 11(1): e0146562.