光声显微成像兼具高光学组织对比度，以及超声的高穿透深度和高空间分辨率的优点。但一方面在光声显微成像中，组织深层目标易被浅表目标屏蔽，另一方面其横向分辨率与焦深的矛盾一直限制了光声显微的成像质量。针对这两个问题，本实验搭建了一套基于超声分辨的光声显微成像系统。其采用一种非共轴的照明-探测方式来减少浅层组织对深层信息的影响，并且采用一种新的焦区积分算法来进行数据的处理，结合多次纵向变焦扫描来实现深层组织的高分辨探测。仿体实验表明，该方法可以在不同纵向深度上保持约 0.6 mm 的横向分辨率，与理论值相接近。裸鼠肿瘤成像结果表明，本文所提出的方法可以比普通的背向探测声聚焦光声显微成像方法在深度方向上获取更多的信息。随着超快脉冲激光和高速扫描方式的发展，本文所提出的方法有望在脑部血管成像、宫颈癌内窥成像、浅表肿瘤成像等方面得到较好的应用
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