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-  2018 

高频电刺激改变神经元发放与场电位节律之间的锁相关系

DOI: doi:10.7507/1001-5515.201706073

Keywords: 高频电刺激, 锋电位, 节律性发放, θ 节律, 锁相

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Abstract:

临床上,已将深部脑刺激(DBS)技术成功应用于治疗多种脑部疾病。近年的研究推测,DBS 技术使用的电脉冲高频刺激(HFS)可以改变神经元动作电位的病理性节律发放,这可能是 DBS 治疗脑部疾病的重要机制之一,但是这种推测尚需实验数据的证实。本文在已麻醉的大鼠海马 CA1 区的输入轴突束薛氏侧支(Schaffer collaterals)施加时长为 1 min、频率为 100 Hz 的 HFS,并分析刺激下游 CA1 区的锥体神经元和中间神经元的锋电位,考察两种神经元的节律性动作电位发放的变化情况。结果显示,麻醉大鼠 CA1 区的场电位存在明显的 θ 节律,且在顶树突层尤其明显,而神经元锋电位与 θ 节律存在锁相关系。与此基线记录相比较,在 HFS 期间,锥体神经元的锋电位与顶树突层 θ 节律的锁相值从 0.36 ± 0.12 显著减小至 0.06 ± 0.04(P < 0.001,配对 t 检验,N = 8);中间神经元锋电位的锁相值也从 0.27 ± 0.08 减小至 0.09 ± 0.05(P < 0.01,配对 t 检验,N = 8)。两类神经元与胞体层 θ 节律的锁相关系也有类似改变。此结果表明,对轴突束施加 HFS 可以消除下游神经元动作电位发放与场电位 θ 节律之间的锁相关系,改变神经元的节律性发放模式。这种现象产生的机制可能是 HFS 诱导的轴突传导障碍,此发现对于深入研究 DBS 的作用机制具有重要的意义

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