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-  2015 

动物源性生物材料中残留α-Gal抗原检测方法

DOI: doi:10.7507/1001-5515.20150120

Keywords: 动物源性生物材料, α-Gal抗原, ELISA抑制法, M86抗体

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Abstract:

为了定量检测动物源性生物材料中残留α-Gal抗原含量, 本实验室建立了M86抗体的ELISA抑制法。用兔血红细胞制备标准曲线, 以Galα-1, 3-Gal/BSA为固相抗原, 利用特异性抗α-Gal单抗M86与待测物反应, 再取上清液中剩余的M86抗体与固相抗原反应后检测待测物中α-Gal抗原含量。结果显示其标准曲线的R2=0.999, 具有很好的相关性; 经α-Gal阳性和阴性材料的验证证实其具有较好的灵敏性和特异性。利用该方法考察了大鼠组织、动物源性脱细胞生物材料、猪真皮处理前后α-Gal抗原的表达特征。该方法有望成为评价动物源性生物材料及其衍生物α-Gal抗原残留的一种有效方法

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