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-  2015 

聚乳酸/乙醇酸膜预防兔腹腔术后粘连的实验研究

DOI: doi:10.7507/1001-5515.20150027

Keywords: 聚乳酸/乙醇酸膜, 胶原纤维, 转化生长因子-β1, 腹腔术后粘连

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Abstract:

为探讨聚乳酸/乙醇酸(PLGA)膜对兔腹腔术后粘连的预防作用及其机制。将66只日本大耳白兔随机分成正常对照组、模型对照组和PLGA膜组。除正常对照组外, 其余各组均采用多因素法制作兔腹腔术后粘连模型, PLGA膜组创面贴覆PLGA膜, 模型对照组创面不处理, 并在不同时间点测定血常规、血浆肝肾功能和纤维蛋白原含量, 分别在术后1、2、4、6、12周时, 处死动物并评定腹腔粘连程度。取盲肠组织行Masson染色和转化生长因子-β1(TGF-β1)免疫组化法, 观察胶原纤维的增生和TGF-β1阳性表达情况。术后粘连程度显示PLGA膜组明显低于模型对照组, 并对肝肾功能、血常规指标均无明显影响, 但分别在术后1周和2周能显著降低纤维蛋白原含量和白细胞数目, 并且胶原纤维面密度和TGF-β1的总光密度均显著低于模型对照组。结果表明, PLGA膜可有效预防兔腹腔术后粘连, 其与减少纤维蛋白原的渗出, 抑制胶原纤维组织的增生和TGF-β1的过表达有关

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