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-  2015 

重症急性胰腺炎大鼠肠黏膜屏障及肠道菌群的变化

DOI: doi:10.7507/1001-5515.20150074

Keywords: 重症急性胰腺炎, 肠黏膜屏障, 肠道微生态, 实时荧光定量-聚合酶链式反应

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Abstract:

本文旨在探讨重症急性胰腺炎(SAP)大鼠肠黏膜屏障、肠道菌群的变化和细菌移位的情况。将健康雄性SD大鼠随机分为对照组(n=10)和实验组(n=14), 采用5%牛磺胆酸钠胰胆管逆行注射法制作重症急性胰腺炎模型, 24 h后处死, 测定血清淀粉酶及血浆内毒素水平, 观察胰腺、小肠病理改变并评分, 检测腹腔脏器细菌移位率, 实时荧光定量聚合酶链式反应法(RT-PCR)定量分析肠道内菌群数量。结果显示:实验组大鼠肠腔内大肠埃希菌属计数明显升高(9.72±3.58, P<0.01), 乳酸杆菌属(0.67±0.34, P<0.01)和双歧杆菌属(4.59±3.42, P<0.05)计数明显减少, 血浆内毒素测定阳性率增高(P<0.01), 腹腔脏器细菌移位率升高(P<0.01或P<0.05), 胰腺及小肠出现明显病理损伤。表明重症急性胰腺炎大鼠肠黏膜屏障受损, 出现肠道菌群失调、细菌及内毒素移位, 其与多脏器感染的发生、发展或有关联

References

[1]  1. LIU Zhonghui, PENG Junsheng, LI Chujun, et al. A simple taurocholate-induced model of severe acute pancreatitis in rats[J]. World J Gastroenterol, 2009, 15(45):5732-5739.
[2]  2. BJORNSON H S. Pancreatic "abscess":diagnosis and management[J]. Pancreas, 1991, 6(Suppl 1):S31-S36.
[3]  3. FREY C F, BRADLEY E L 3rd, BEGER H G. Progress in acute pancreatitis[J]. Surg Gynecol Obstet, 1988, 167(4):282-286.
[4]  4. RUNKEL N F, MOODY F G, SMITH G S, et al. The role of the gut in the development of sepsis in acute pancreatitis[J]. J Surg Res, 1991, 51(1):18-23.
[5]  5. KAZANTSEV G B, HECHT D W, RAO R, et al. Plasmid labeling confirms bacterial translocation in pancreatitis[J]. Am J Surg, 1994, 167(1):201-206; discussion 206-207.
[6]  6. 郝建志, 方驰华, 范应方, 等.一种效果确切的大鼠重症急性胰腺炎模型[J].中国组织工程研究与临床康复, 2011, 15(28):5193-5196.
[7]  7. 何志宇, 陈光远.重症急性胰腺炎与胃肠动力障碍的研究进展[J].世界华人消化杂志, 2008, 16(12):1317-1322.
[8]  8. BESSELINK M G, VAN SANTVOORT H C, RENOOIJ W, et al. Intestinal barrier dysfunction in a randomized trial of a specific probiotic composition in acute pancreatitis[J]. Ann Surg, 2009, 250(5):712-719.
[9]  9. SCHMIDT J, RATTNER D W, LEWANDROWSKI K, et al. A better model of acute pancreatitis for evaluating therapy[J]. Ann Surg, 1992, 215(1):44-56.
[10]  10. SCHMIDT J, LEWANDROWSI K, WARSHAW A L, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat[J]. Int J Pancreatol, 1992, 12(1):41-51.
[11]  11. 田华, 张喜平, 陈力, 等.重症急性胰腺炎大鼠模型制备的技术改良[J].中华急诊医学杂志, 2005, 14(3):211-214.
[12]  12. CHIU C J, MCARDLE A H, BROWN R, et al. Intestinal mucosal lesion in low-flow states.Ⅰ. A morphological, hemodynamic, and metabolic reappraisal[J]. Arch Surg, 1970, 101(4):478-483.
[13]  13. 杨荣, 嵇武, 李国逊, 等.粪便中肠源性防御素-5含量变化对腹腔感染大鼠肠黏膜屏障功能监测的意义[J].中国现代医学杂志, 2011, 21(11):1292-1296.
[14]  14. 宋关茹, 姚萍.应用实时荧光定量PCR定量检测溃疡性结肠炎肠道大肠埃希菌、乳酸杆菌及双歧杆菌属的变化[J].中国微生态学杂志, 2012, 24(3):239-243.
[15]  15. RINTTIL? T, KASSINEN A, MALINEN E, et al. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR[J]. J Appl Microbiol, 2004, 97(6):1166-1177.
[16]  16. OHLAND C L, MACNAUGHTON W K. Probiotic bacteria and intestinal epithelial barrier function[J]. Am J Physiol Gastrointest Liver Physiol, 2010, 298(6):G807-G819.
[17]  17. ROMOND M B, COLAVIZZA M, MULLIé C, et al. Does the intestinal bifidobacterial colonisation affect bacterial translocation?[J]. Anaerobe, 2008, 14(1):43-48.
[18]  18. SHIMIZU K, OGURA H, GOTO M, et al. Altered gut flora and environment in patients with severe SIRS[J]. J Trauma, 2006, 60(1):126-133.
[19]  19. MAZAKI T. Re:early antibiotic treatment for severe acute necrotizing pancreatitis[J]. Ann Surg, 2008, 247(2):393-394; author reply 394-395.
[20]  20. BALZAN S, DE ALMEIDA QUADROS C, DE CLEVA R, et al. Bacterial translocation:overview of mechanicsms and clinical impact[J]. J Gastroenterol Hepatol, 2007, 22(4):464-471.
[21]  21. 袁耀宗, 汤玉茗.重视肠屏障功能的研究[J].中华消化杂志, 2006, 26(9):577-578.
[22]  22. 邓群, 黎沾良, 陆连荣, 等.急性坏死性胰腺炎时肠屏障损害及肠源性细菌和内毒素移位的实验研究[J].中国普通外科杂志, 2001, 10(4):313-316.
[23]  23. QUIGLEY E M, QUERA R. Small intestinal bacterial overgrowth:roles of antibiotics, prebiotics, and probiotics[J]. Gastroenterology, 2006, 130(2 Suppl 1):S78-S90.

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