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-  2015 

γ-分泌酶抑制剂DAPT对神经前体细胞系分化的作用研究

DOI: doi:10.7507/1001-5515.20150023

Keywords: DAPT, 神经元前体细胞系, 神经元, 分化

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Abstract:

本研究目的为探究γ-分泌酶抑制剂DAPT(3, 5-二氟苯乙酰-L-丙氨酰-S苯基甘氨酸t-丁酯)对神经元前体细胞系分化的影响, 检验经DAPT诱导产生更多可用于移植的神经元的可能性。培养神经元前体细胞系GE6时, 以培养基中加入4μmol/L DAPT的为实验组, 不加DAPT的为对照组, 分化4 d, 采用免疫荧光染色的方法分别检测两组细胞Tuj1、GFAP、O4的阳性率, 采用qRT-PCR分别检测两组细胞Tuj1 mRNA、GFAP mRNA相对表达量。免疫荧光染色表明实验组Tuj1阳性率较对照组增加, 而GFAP和O4的阳性率较对照组减少, 这些差异均有统计学意义(P<0.05)。qRT-PCR中两组Tuj1和GFAP mRNA的变化趋势与免疫荧光染色结果一致。结果提示DAPT能促进神经元前体细胞系向神经元分化, 抑制其向胶质细胞分化, 可以用DAPT诱导产生更多的可用于移植的神经元

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