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-  2016 

可植入光遗传学神经刺激光极研究

DOI: doi:10.7507/1001-5515.20160057

Keywords: 光遗传学, 神经刺激, 光极

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Abstract:

本文为实现多点神经环路、核团功能的在体调控, 研发了可植入光遗传学神经刺激光极。首先, 使用了柔性电路板工艺制作光极的基底, 将微型发光二极管焊接于基底之上后使用真空气相镀膜技术制作光极的覆盖膜。然后使用光功率计等设备对光极进行性能测试, 结合动物实验实现光极的验证。最终制作完成的光极尺寸为500 μm×150 μm(宽度×基底厚度); 将其置于生理盐水14 d后仍可正常发光; 最大发光功率为9.31 mW, 有效光照面积达到3.03 mm2; 在体实验中检测到了光刺激诱发的小鼠皮层响应, 并成功调控了小鼠的次级运动皮层。本文研制的光极具有光照面积大、植入方便、可长期植入等特点, 为核团功能调控等研究提供了新的光遗传学工具

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