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-  2017 

18氟-氟代脱氧葡萄糖与 18氟-氟代胸腺嘧啶用于吉西他滨联合顺铂治疗非小细胞肺癌动物模型的早期疗效监测比较

DOI: doi:10.7507/1001-5515.201610011

Keywords: 18 氟-氟代脱氧葡萄糖, 18 氟-氟代胸腺嘧啶, 非小细胞肺癌, 疗效监测, 吉西他滨, 顺铂

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Abstract:

非小细胞肺癌(NSCLC)约占肺癌的 80% 以上,吉西他滨联合顺铂已成为目前治疗 NSCLC 的一线化疗方案。本研究旨在探讨 18氟-氟代脱氧葡萄糖(18F-FDG)和 18氟-氟代脱氧胸腺嘧啶(18F-FLT)的小动物正电子(microPET)显像早期监测吉西他滨联合顺铂治疗 NSCLC 疗效的价值。以荷 Lewis 肺癌的 C57BL/6 小鼠作为动物模型,实验组给予吉西他滨加顺铂联合化疗,对照组给予等量生理盐水处理。分别在治疗前和治疗后第 3 天进行 18F-FDG 和 18F-FLT 的 microPET 显像,观察实验组及对照组动物肿瘤体积及 18F-FDG、18F-FLT 摄取的变化。显像结束后处死部分小鼠,取肿瘤组织进行苏木精-伊红(HE)染色及抗原 Ki67 免疫组化染色。与对照组相比,实验组的肿瘤生长明显受到抑制(P<0.05)。在实验组中,18F-FLT 的最大标准化摄取值(SUVmax)从治疗前的 0.59±0.05 下降至治疗后第 3 天的 0.28±0.05,结果显示差异具有统计学意义(P<0.05),而 18F-FDG 的 SUVmax 从治疗前的 4.35±0.46 下降至治疗后第 3 天的 4.02±0.47,结果显示差异不具有统计学意义(P>0.05)。组织学结果显示,在采用吉西他滨加顺铂治疗后,肿瘤增殖指标抗原 Ki67 出现明显下降(P<0.05)。HE 染色提示化疗后肿瘤组织中有较多坏死细胞及炎性细胞。本研究结果提示,18F-FLT 比 18F-FDG 能够更早期监测吉西他滨联合顺铂治疗 NSCLC 的效果;此外,18F-FLT 受化疗后肿瘤组织中的炎症反应干扰更小

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