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-  2015 

衰老标记蛋白30在慢性阻塞性肺疾病患者中的表达及意义

DOI: doi:10.7507/1671-6205.2015085

Keywords: 衰老标记蛋白30, 肺泡巨噬细胞, 慢性阻塞性肺疾病

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Abstract:

目的初步探索衰老标记蛋白30(SMP-30)在人肺组织中的表达以及在慢性阻塞性肺疾病(慢阻肺)发病中的意义。 方法纳入2012年1月至6月在江苏省人民医院因肺部肿瘤行肺叶切除术的患者20例。肺组织标本从江苏省人民医院组织库申请获得, 为距离癌组织>5 cm的相对正常组织。根据患者肺功能及吸烟情况, 将患者分为健康对照组7例, 吸烟对照组7例, 以及慢阻肺组6例。采用免疫组织化学和蛋白免疫印迹法检测SMP-30在各组肺组织中的细胞定位及含量。 结果SMP-30在人肺组织中主要分布于肺泡巨噬细胞胞浆, 慢阻肺组肺泡巨噬细胞及SMP-30阳性细胞数量均明显增多。蛋白免疫印迹法显示肺组织SMP-30蛋白在吸烟非慢阻肺组的含量明显高于健康对照组(2.16±0.23比1.10±0.14, P<0.01)。相较于吸烟非慢阻肺组, 慢阻肺组SMP-30蛋白表达进一步增加(4.62±0.97比2.16±0.23, P<0.05)。 结论SMP-30的表达增加可能与肺泡巨噬细胞寿命延长、数量增多有关, 有可能是慢阻肺发生发展的一个病理环节

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