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-  2015 

哮喘及呼吸道合胞病毒感染小鼠的神经生长因子、白血病抑制因子的表达及地塞米松的影响

DOI: doi:10.7507/1671-6205.2015038

Keywords: 呼吸道合胞病毒, 神经生长因子, 白血病抑制因子, 哮喘, 地塞米松

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Abstract:

目的探讨神经生长因子(NGF)、白血病抑制因子(LIF)在支气管哮喘小鼠及呼吸道合胞病毒(RSV)感染小鼠的表达,两者是否存在相同的神经源性机制,寻找治疗哮喘的新靶点。 方法100只健康Balb/c纯系小鼠随机分为5组:正常对照组,RSV组,哮喘组,哮喘合并RSV组,地塞米松组。HE染色观察肺组织病理变化,RT-PCR技术进行肺组织NGF mRNA及LIF mRNA的定量分析,免疫组织化学方法检测NGF蛋白及LIF蛋白。 结果RSV组镜下可见肺泡隔增宽,肺泡上皮肿胀,间质水肿。哮喘组及哮喘合并RSV组小鼠肺泡间隔增宽,支气管管腔变小,管壁增厚,小血管、细支气管及肺泡周围可见大量炎症性细胞,上述改变哮喘合并RSV组较哮喘组表现明显,而地塞米松组较RSV组表现减轻,正常对照组无炎症细胞浸润。各组均有NGF mRNA及LIF mRNA、NGF蛋白及LIF蛋白表达,哮喘合并RSV组表达最强,哮喘组及RSV组均较正常对照组表达增多,地塞米松组较RSV组表达减少。 结论RSV感染时肺组织NGF及LIF表达有所增加,合并哮喘时表达显著增加,地塞米松干预对NGF及LIF的表达均有一定的抑制作用

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