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-  2016 

白细胞介素27在呼吸道合胞病毒致肺部感染中的作用机制研究

DOI: doi:10.7507/1671-6205.2016084

Keywords: 白细胞介素27, 呼吸道合胞病毒, STAT1, pSTAT

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Abstract:

目的 研究呼吸道合胞病毒(RSV)感染小鼠中白细胞介素27(IL-27)水平的变化,以及IL-27在离体细胞中对RSV病毒复制的影响。 方法 在动物实验中,取7周龄雌性C57小鼠,分别在RSV感染后的第0、0.5、1、2、4、6、8 d处死小鼠(每组5只),左肺提取RNA,运用Real-time PCR法检测RSV感染不同时间后IL-27p28、IL-27EBI3、RSV-M、干扰素β(IFN-β)mRNA的变化,右肺行HE染色。在细胞实验中,培养人肺腺癌上皮细胞(A549),用不同浓度的重组白细胞介素27(rhIL-27)0、1、 10和50 ng/mL刺激培养并感染RSV 24 h,运用Real-time PCR法检测干扰素诱导跨膜蛋白3(IFITM3)、IFN-β、RSV-M mRNA的变化,运用Western blot法检测IFITM3、STAT1/pSTAT1蛋白的变化。 结果 RSV感染小鼠后第4 d病毒复制达到高峰后逐渐降低,8 d后基本恢复。IFN-β mRNA仅在感染后12 h升高,随后迅速恢复正常。IL-27 p28/EBI3基因在感染后第1 d达高峰后逐渐降低,4 d后基本恢复。在离体试验中,发现10 ng/mL、50 ng/mL浓度的rhIL-27刺激感染的A549细胞可抑制病毒复制,促进IFTIM3基因的表达,并且可以促进STAT1磷酸化。不同浓度的rhIL-27刺激后IFN-β mRNA的表达变化无显著差异。 结论 在RSV感染小鼠后肺组织中IL-27 mRNA表达增高,而IL-27可能通过促进STAT1磷酸化调节IFITM3蛋白的表达,具有抑制RSV复制的作用

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