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-  2016 

慢性阻塞性肺疾病大鼠肺组织自噬相关蛋白的变化

DOI: doi:10.7507/1671-6205.2016110

Keywords: 慢性阻塞性肺疾病, 自噬, 大鼠, 肺组织

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Abstract:

目的研究在慢性阻塞性肺疾病(简称慢阻肺)大鼠肺组织中自噬相关蛋白的变化。 方法单纯烟熏法构建慢阻肺大鼠动物模型,采用Real time PCR,Western blot技术检测检测大鼠肺组织中PI3K、AKT、p-AKT、mTOR、p-mTOR及自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1、P62、Atg7、Atg12的mRNA及蛋白表达,探讨在慢阻肺大鼠肺组织中自噬水平及自噬相关蛋白的变化。用LC3B免疫组化比较COPD组与正常大鼠间自噬水平的变化。 结果Real time PCR分析结果显示,与正常组相比,慢阻肺组大鼠肺组织中自噬相关基因Beclin1、Atg5、Atg12的mRNA表达显著增高( P<0.05,其中Beclin1、Atg5两组比较 P<0.01)。Atg7的mRNA表达在慢阻肺组与正常组之间差异无统计学意义( P>0.05)。Western blot分析结果显示,慢阻肺组大鼠肺组织中PI3K蛋白、p-AKT/AKT及p-mTOR/mTOR蛋白表达显著降低( P<0.05)。自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1蛋白表达增高( P<0.05,其中LC3Ⅱ/Ⅰ、Atg5两组比较 P<0.01)。P62蛋白表达显著下降( P<0.01)。LC3B免疫组化显示,慢阻肺组LC3B表达高于正常组。 结论烟熏12周的慢阻肺大鼠与正常组相比,自噬通路上游蛋白PI3K、p-AKT/AKT、p-mTOR/mTOR的表达降低,自噬蛋白表达增加,自噬水平增加

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