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-  2017 

埃兹蛋白在转化生长因子-β 诱导的支气管上皮细胞间充质转化中的作用

DOI: doi:10.7507/1671-6205.201702015

Keywords: 支气管上皮细胞, 上皮间充质转化, 转化生长因子-β, 埃兹蛋白

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Abstract:

目的探讨埃兹蛋白(ezrin)在支气管上皮细胞(16HBE)间充质转化(EMT)中的作用。 方法建立转化生长因子-β(TGF-β)诱导的 16HBE 的 EMT 模型;RT-PCR 和 Western blot 法检测 16HBE 中 ezrin mRNA 和蛋白表达;通过慢病毒 shRNA 抑制 ezrin 表达,观察细胞形态,检测细胞 EMT 的生物标志分子上皮钙黏着蛋白(E-cadherin)、波形蛋白(vimentin)和 α-平滑肌动蛋白(alpha smooth muscle actin,α-SMA)的 mRNA 和蛋白表达水平;加入外源性 ezrin 蛋白,细胞免疫荧光、RT-PCR 及 Western blot 法检测其对 EMT 标志物的影响。 结果TGF-β 能下调 16HBE 中 ezrin 的 mRNA 和蛋白表达。经 ezrin 敲减后,同 TGF-β 刺激表现相同,16HBE 也由典型的铺路石样变为梭形,同时其 E-cadherin 表达下降,vimentin 和 α-SMA 表达增加;加入重组蛋白 ezrin 后,16HBE 的 E-cadherin 表达增多,vimentin 和 α-SMA 表达下降。 结论TGF-β 可能通过下调 ezrin 表达促进 16HBE 发生 EMT

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