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-  2016 

应用基因芯片筛选转染FOXO1后A549细胞差异表达基因

DOI: doi:10.7507/1671-6205.2016087

Keywords: 叉头蛋白O1, 基因芯片, A549细胞, 急性肺损伤

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Abstract:

目的 研究转染叉头蛋白O1(FOXO1)后A549细胞基因表达谱的改变,为深入探讨FOXO1在急性肺损伤中的作用机制提供线索。 方法 应用肿瘤坏死因子α(TNF-α)刺激A549细胞,应用脂质体介导的转染方法将真核表达载体GV230-FOXO1转染入经TNF-α诱导的A549细胞,再提取细胞的总RNA,应用基因芯片技术进行分析。 结果 成功构建并验证了FOXO1真核表达载体,提取了高质量的mRNA进行芯片分析,并通过RNA质量控制(RNA QC)验证。芯片分析结果显示差异表达基因中上调的基因有317个,下调的基因有237个。这些差异表达的基因功能涉及细胞增殖、凋亡、分化等多个方面。 结论 应用基因芯片技术可成功筛选转染FOXO1基因后A549细胞的差异表达基因。FOXO1在急性肺损伤中可能通过改变细胞的增殖、凋亡与分化等水平来影响疾病的进程

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