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-  2017 

分泌型卷曲相关蛋白 1 在慢性阻塞性肺疾病气道重塑中的作用和机制

DOI: doi:10.7507/1671-6205.201702038

Keywords: 慢性阻塞性肺疾病, 分泌型卷曲相关蛋白 1, 支气管上皮细胞

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Abstract:

目的 研究分泌型卷曲相关蛋白 1(SFRP1)在慢性阻塞性肺疾病(简称慢阻肺)气道重塑中的作用和机制。 方法 纳入 2014 年 6 月至 2016 年 6 月在江苏省人民医院确诊慢阻肺的稳定期患者 45 例,健康对照 21 例。采取酶联免疫吸附试验检测血清 SFRP1 的表达,采用蛋白印迹法检测人支气管上皮细胞(16HBE)的 SFRP1、上皮钙黏素(E-cadherin)及 α-平滑肌肌动蛋白(α-SMA)的表达。 结果 慢阻肺患者血清 SFRP1 水平较健康对照明显升高 [(80.65±15.01)pg/ml 比(23.89±2.59)pg/ml, P<0.05],慢阻肺患者血清 SFRP1 水平与肺功能(FEV 1、FEV 1%pred、FVC)呈负相关(相关系数 r 分别为 –0.368 9、–0.382 6、–0.417 3, P<0.05)。慢阻肺吸烟组患者血清 SFRP1 水平明显高于慢阻肺不吸烟组[(97.74 ± 21.95) pg/ml 比(52.51 ± 14.87)pg/ml, P<0.05]。香烟烟雾提取物可以刺激 16HBE 细胞表达 SFRP1,外源性 SFRP1 能下调 E-cadherin 的表达,并上调 α-SMA 的表达。 结论 SFRP1 可能通过调控支气管上皮细胞 E-cadherin 和α-SMA 参与慢阻肺气道重塑的形成

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