目的观察水通道蛋白 1（AQP1）对内皮祖细胞（EPC）增殖迁移功能的影响。 方法体外分离 AQP1 野生型（WT）（ n=6）和敲除型（KO）小鼠（ n=6）骨髓细胞并定向培养分化为 EPC。应用免疫荧光法检测细胞表面抗原鉴定 EPC，通过无标记活细胞动态分析技术、细胞 Transwell 迁移实验及划痕实验比较 AQP1 WT 和 KO 小鼠中 EPC 的功能差异。 结果对小鼠 EPC 培养观察发现，细胞最初保持悬浮状态，7 d 内逐渐黏附贴壁成典型的间充质干细胞，间充质干细胞经内皮细胞专用培养基培养 7 d 后逐渐分化贴壁，14 d 后细胞继续增殖，形态为梭形或多边形，并融合成铺路石样的 EPC 细胞。细胞表面标志鉴定发现，应用内皮细胞专用培养基培养 7 d 后细胞 CD133 及 CD31 表达阳性，随着培养时间的延长在 14 d 时 CD34 及 Flk-1 表达阳性。免疫荧光法验证结果显示，AQP1 仅在 AQP1 WT 小鼠的 EPC 中表达阳性。对不同 AQP1 状态下 EPC 的功能研究结果显示，培养 72 h 的 AQP1 WT 小鼠与 KO 小鼠 EPC 细胞增殖数目无显著差异。Transwell 检测结果显示，AQP1 KO 小鼠其 EPC 迁移能力较 WT 小鼠明显减弱。细胞划痕实验检测结果显示，AQP1 KO 小鼠 EPC 的划痕愈合能力也明显低于 WT 小鼠。 结论EPC 最初表现出干细胞特征，随着培养时间延长，逐渐表现出内皮细胞特征。AQP1 可影响 EPC 的迁移能力而非增殖能力
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