目的研究 CD73 在间歇性低氧合并高脂饮食条件下大鼠心脏损伤中的表达及意义。 方法建立大鼠慢性间歇性低氧合并高脂饮食模型。将 SPF 级健康雄性 Wistar 大鼠 24 只随机分为 4 组，每组 6 只。A 组（常氧及普通饮食）；B 组（常氧及高脂饮食）；C 组（间歇性低氧及普通饮食）；D 组（间歇性低氧及高脂饮食）。在实验 6 周后，取各组大鼠血清检测血脂（甘油三酯、低密度脂蛋白胆固醇）水平，光镜下观察大鼠心肌形态学变化，免疫组化和蛋白印迹检测 CD73 蛋白在各组大鼠心肌细胞中的表达水平。 结果4 组大鼠血脂水平比较，差异有统计学意义（均 P<0.05）。HE 结果显示：A 组心肌细胞未见明显异常； B 组和 C 组中可见部分心肌纤维排列紊乱，局灶性变性坏死；D 组心肌细胞损伤最为明显，可见肌纤维排列紊乱，纵横纹不清，部分肌纤维明显溶解。免疫组化显示，与 A 组比较，B、C、D 组心肌细胞 CD73 蛋白表达水平均明显升高（均 P<0.01）；与 B 组和 C 组比较，D 组 CD73 蛋白表达水平明显升高（均 P<0.01）。蛋白印迹也显示出一致的结果：与 A 组比较，B、C 组 CD73 蛋白表达水平均显著升高（均 P <0.05），D 组 CD73 蛋白表达水平升高更为显著（ P<0.01）；与 B 组和 C 组比较，D 组 CD73 蛋白表达水平明显升高（均 P <0.01）。 结论慢性间歇性低氧、高脂饮食可以引起心肌细胞的损伤和心肌细胞中 CD73 表达上调
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