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-  2017 

白藜芦醇对哮喘小鼠气道重塑的抑制作用

DOI: doi:10.7507/1671-6205.201607027

Keywords: 哮喘, 气道重构, 白藜芦醇, 小鼠模型

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Abstract:

目的观察白藜芦醇(RV)对慢性哮喘小鼠模型气道重塑的影响。 方法24 只雌性 BALB/c 小鼠随机分成 3 组,即对照组、哮喘组、RV 组,每组 8 只,3 组小鼠均给予鸡卵清蛋白(OVA)致敏,对照组予以磷酸盐缓冲溶液(PBS)激发,哮喘组和 RV 组予以 OVA 激发,其中 RV 组于每次激发前 30 min 给予腹腔注射溶于二甲基亚砜(DMSO)的 RV,对照组和哮喘组给予腹腔注射等量的 DMSO。最后一次激发 24 h 后,取小鼠肺组织固定、切片行过碘酸希夫(PAS)染色、Masson 染色及 α-平滑肌肌动蛋白(α-SMA)免疫组织化学检测,分别检测杯状细胞增生、胶原沉积和平滑肌细胞增生肥大程度,结果用杯状细胞(PAS 阳性)、胶原(Masson 阳性)和平滑肌细胞(α-SMA 阳性)阳染面积/支气管基底膜周长(μm 2/μm)表示。 结果对照组未见 PAS + 区域,哮喘组和 RV 组杯状细胞增生显著高于对照组(5.44±1.13、4.18±0.85 比0.00±0.00, P 均<0.01),且 RV 组显著低于哮喘组( P<0.05)。Masson 染色提示哮喘组和 RV 组上皮下胶原沉积显著高于对照组(9.80±2.78、5.71±0.68 比 1.67±0.65,均 P<0.01),且 RV 组显著低于哮喘组( P<0.01)。α-SMA 免疫组化显示哮喘组和 RV 组上皮下胶原沉积显著高于对照组(10.39±1.65、7.57±1.98 比 2.41±1.06,均 P<0.01),且 RV 组显著低于哮喘组( P<0.05)。 结论RV 干预后,小鼠慢性哮喘模型气道重塑指标均有减轻,提示 RV 可能对慢性哮喘小鼠气道重塑具有抑制作用

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