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-  2017 

辛伐他汀早期与后期干预野百合碱诱导肺动脉高压大鼠的研究

DOI: doi:10.7507/1671-6205.201606049

Keywords: 肺动脉高压, 辛伐他汀, 野百合碱, 血管重构, 预防

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Abstract:

目的 观察不同时期应用辛伐他汀对野百合碱(MCT)诱导大鼠肺动脉高压(PAH)的改善作用,初步评价早期应用辛伐他汀对 PAH 的预防效果。 方法 将 24 只 SD 大鼠随机分成 4 组,每组 6 只。对照组:d 0 腹腔注射生理盐水 1 次;PAH 模型组:d 0 腹腔注射 MCT(50 mg/kg)1 次;早期干预组:腹腔注射 MCT(50 mg/kg)d –7~d –1 辛伐他汀(20 mg·kg –1·d –1)灌胃,d 0 腹腔注射MCT(50 mg/kg)1 次,d 1~14 辛伐他汀灌胃;后期干预组:d 0 腹腔注射 MCT(50 mg/kg),d 15~35 辛伐他汀(20 mg·kg –1·d –1)灌胃。d 36 通过右心导管测大鼠右心室收缩压(RVSP)和平均肺动脉压(mPAP),处死大鼠后分离心肺,测定右心室肥厚指数(RVHI)和肺小动脉中膜厚度百分比(WT%),进行肺小动脉周围炎症评分。 结果 与 PAH 组比较,早期干预组及后期干预组 RVSP、mPAP、RVHI、WT% 得到显著改善( P<0.01),肺小动脉周围炎症评分降低( P<0.05);与后期干预组比较,早期干预组 mPAP、RVSP 改善更明显( P<0.05),WT% 下降更显著( P<0.01),而 RVHI、肺小动脉周围炎症评分无显著差异( P>0.05)。 结论 辛伐他汀早期及后期干预均可改善 MCT 诱导 PAH 大鼠 RVSP、mPAP 及 WT% 的恶化,早期干预较后期干预效果更显著

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