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-  2017 

普通肝素改善高迁移率族蛋白 1 介导的血管内皮细胞屏障通透性增加的实验研究

DOI: doi:10.7507/1671-6205.201607045

Keywords: 脓毒症, 高迁移率族蛋白 1, 普通肝素, 胞质紧密粘连蛋白-1, 核因子-κB

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Abstract:

目的 观察普通肝素(UFH)对高迁移率族蛋白 1 (HMGB 1 )介导的人脐静脉内皮细胞屏障通透性损伤的保护作用,探讨 UFH 对 HMGB 1 介导的胞质紧密粘连蛋白 -1 (ZO-1)表达缺失的保护机制。 方法 将人脐静脉内皮细胞进行体外培养,人脐静脉内皮细胞株传代培养后分为 4 组( n = 5 ):空白对照组(加入等量 PBS)、HMGB 1 处理组(100 ng/ml)、UFH 对照组(UFH 10 U/ml)、HMGB 1 及 UFH 处理组(100 ng/ml HMGB 1 + UFH 10 U/ml)。MTT 法测定内皮细胞存活率,用 Transwell 小室法测定单层内皮屏障通透性,用免疫荧光染色法测定 ZO-1 的表达分布,蛋白免疫印迹(Western blot)法检测 ZO-1 蛋白及核因子 -κB(NF-κB)的表达。 结果 HMGB 1 (100 ng/ml)对内皮细胞活性无抑制作用( P>0.05)。UFH 预处理后可减少 HMGB1 所致的内皮细胞通透性增加( P<0.05)。UFH 预处理后可降低 HMGB 1 所致内皮细胞 ZO-1 密闭环的减少和破坏,使 ZO-1 荧光强度增强,ZO-1 蛋白表达增加,并使 NF-κB 的核易位减少。 结论 UFH可保护 HMGB 1 介导的内皮细胞 ZO-1 的表达缺失,进而改善内皮细胞屏障通透性,其机制与减少 NF-κB 核易位相关

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