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-  2015 

程序性细胞死亡因子配体1在轻型和重型脓毒症小鼠单核细胞的表达和差异

DOI: doi:10.7507/1671-6205.2015089

Keywords: 脓毒症, 程序性细胞死亡因子配体1, 单核细胞

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Abstract:

目的观察脓毒症小鼠外周血单核细胞程序性细胞死亡因子配体1(PD-L1)的表达及其在轻型、重型脓毒症组间的差异。 方法30只C57小鼠随机分为轻型脓毒症组、重型脓毒症组及假手术组, 每组10只, 采用盲肠结扎穿孔法造模, 以盲肠结扎长短区分病情轻重, 造模成功后观察生存率。60只C57小鼠随机分为轻型脓毒症组、重型脓毒症组及假手术组, 每组均为20只。分别在造模后6 h、24 h时间点取外周血, 每个时间点10只。流式细胞技术检测各组单核细胞PD-L1的表达, 酶联免疫吸附法检测血清肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)水平。 结果重型脓毒症组较轻型脓毒症组死亡率高。6 h、24 h脓毒症组血清TNF-α和IL-10水平较假手术组明显升高(P<0.01)。6 h和24 h脓毒症组单核细胞PD-L1表达较假手术组升高(P<0.05), 24 h脓毒症组PD-L1表达升高更明显(P<0.01)。重型脓毒症组PD-L1表达较轻型脓毒症组有升高趋势, 但组间差异无统计学意义(P>0.05)。 结论脓毒症小鼠单核细胞PD-L1高表达, 重型脓毒症组较轻型脓毒症组有升高趋势

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