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-  2017 

高糖经由 ROS 或 TGF-β1/PI3K/Akt 信号途径促进肺腺癌 A549 细胞 HO-1 表达增加

DOI: doi:10.7507/1671-6205.201605052

Keywords: 血红素加氧酶1, 人肺上皮细胞, 高糖, 活性氧

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Abstract:

目的 探讨高糖对肺腺癌 A549 细胞血红素加氧酶 -1(HO-1)表达的影响及机制。 方法 应用 Western blot 技术和逆转录 PCR 技术检测高糖对人肺腺癌上皮细胞系 A549 细胞 HO-1 的表达。应用酶联免疫吸附试验检测 HO-1 酶活性和氧化应激产物。 结果 用 25 mmol/L 高糖处理肺 A549 细胞 0、24、48、72 h,以及用 5、10、25、40 mmol/L 葡萄糖处理 A549 细胞 48 h,在蛋白水平和 RNA 水平,高糖诱导肺 A549 细胞 HO-1 表达呈浓度和时间依赖性。高糖诱导 A549 细胞活性氧(ROS)和转化生长因子β 1(TGF-β 1)产生增加,并介导 HO-1 表达增加。伴随 HO-1 表达增加,HO-1 酶活性也相应增加。抗氧化剂 N-乙酰半胱氨酸(NAC)和 PI3K/Akt 抑制剂可抑制高糖诱导的肺上皮细胞 HO-1 表达。 结论 高糖促进肺上皮细胞 ROS 和 TGF-β 1 产生,介导 HO-1 表达增加,并伴随 HO-1 酶活性增加

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