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-  2018 

哮喘患者外周血程序性细胞死亡因子-1 的表达研究

DOI: doi:10.7507/1671-6205.201706008

Keywords: 哮喘, 程序性细胞死亡因子-1, γ 干扰素, 白细胞介素-4, 辅助性T细胞

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Abstract:

目的探讨哮喘患者外周血血清程序性细胞死亡因子-1(PD-1)、γ 干扰素(IFN-γ)、白细胞介素-4(IL-4)及辅助性 T 细胞(Th 细胞)的表达。方法以江苏省连云港市第一人民医院呼吸内科收治的 41 例哮喘患者为研究对象,依据病情分为急性发作组(20 例)和慢性持续组(21 例),另选 18 例健康者作为对照组。患者在起病入院时抽取外周血,采用酶联免疫吸附法检测 PD-1、IFN-γ、IL-4 的浓度,流式细胞仪检测外周血 Th1、Th2 及 Th1/Th2 的表达。结果与健康对照组比较,慢性持续组、急性发作组外周血 PD-1、IFN-γ 浓度降低,IL-4 浓度升高,Th1 细胞比率、Th1/Th2 比值降低,Th2 细胞比率升高,差异均有统计学意义(均 P<0.05)。与慢性持续组比较,急性发作组外周血 PD-1、IFN-γ 浓度更低,IL-4 浓度更高,Th1 细胞比率、Th1/Th2 比值更低,Th2 细胞比率更高,差异均有统计学意义(均P<0.05);急性发作组 PD-1 与 IFN-γ、Th1 呈正相关(r 值分别为 0.678 和 0.712,均 P<0.05),与 IL-4、Th2 呈负相关(r 值分别为 –0.745 和 –0.700,均 P<0.05)。结论哮喘患者外周血 PD-1 降低,急性发作组更低,且与常规的哮喘检测指标呈相关性

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