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-  2015 

诱导多能干细胞体外分化为雪旺细胞的研究进展

DOI: doi:10.7507/1002-1892.20150332

Keywords: 诱导多能干细胞, 神经嵴, 雪旺细胞, 分化, 鉴定

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Abstract:

目的对近年诱导多能干细胞(induced pluripotent stem cells,iPS)体外诱导分化为雪旺细胞的相关研究进展进行综述。 方法查阅目前iPS体外诱导分化为雪旺细胞的相关文献,对iPS体外分化为雪旺细胞的诱导方案以及对分化形成的雪旺细胞进行鉴定和功能性检测方法进行总结。 结果研究表明iPS可诱导分化为雪旺细胞,但目前需要先将iPS诱导分化为神经嵴细胞或神经嵴干细胞,再将其进一步分化为雪旺细胞。S100-β和胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)为公认的雪旺细胞标记物。当神经嵴细胞或神经嵴干细胞分化前标记为p75+、HNK1+或nestin+,诱导后标记为S100-β+、GFAP+,表明诱导产生的是雪旺细胞。 结论将iPS诱导分化为雪旺细胞的研究虽然已有进展,但目前成功的诱导方案甚少,仍需对其进行深入研究

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