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-  2017 

联合应用 BMP-2 及细胞分化抑制因子 1 基因延缓兔腰椎间盘退变的动物实验研究

DOI: doi:10.7507/1002-1892.201609035

Keywords: 椎间盘退变, 基因治疗, BMP-2, 细胞分化抑制因子 1

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Abstract:

目的 研究兔腰椎间盘髓核内注射慢病毒携带的 BMP-2 和细胞分化抑制因子 1(inhibitor of differentiation 1,Id1)基因能否有效改善椎间盘退变进程。 方法 取 4 月龄新西兰兔 32 只,体质量 2.0~2.5 kg;以 L3、4、L4、5、L5、6 为目标椎间盘,经腹细针穿刺法制造椎间盘退变模型。将 30 只造模成功的实验动物随机分为 5 组,每组 6 只;造模后 4 周于各组目标椎间盘中分别注射 Lv-BMP-2 病毒(A 组)、Lv-BMP-2+Lv-Id1 病毒(B 组)、Lv-Id1 病毒(C 组)、Lv-绿色荧光蛋白空载体病毒(D 组)及 PBS(E 组)。于注射后 2、4、8 周各组分别随机取 2 只动物行 T2-mapping MRI 检查获得确切的T2弛豫时间(T2 值);之后处死动物取椎间盘组织,应用实时荧光定量 PCR、ELISA 法检测 Ⅱ 型胶原及蛋白聚糖的 mRNA 相对表达量及含量。 结果 T2-mapping MRI 检查示,注射后 0、2 周各组间 T2 值比较差异均无统计学意义(P>0.05);注射后 4 周,A、B 组 T2 值显著高于 C、D、E 组(P<0.05),A、B 组间比较差异无统计学意义(P>0.05);注射后 8 周,B 组 T2 值显著高于其余各组(P<0.05)。实时荧光定量 PCR 及 ELISA 检测示,注射后 2、4、8 周 B 组 Ⅱ 型胶原及蛋白聚糖的 mRNA 相对表达量及含量均显著高于其余各组(P<0.05)。 结论 联合应用 BMP-2 和 Id1 基因在体内实验中能有效延缓兔腰椎间盘退变的进程

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