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-  2015 

富白细胞和血小板血浆对BMSCs在兔股骨头缺血性坏死中成骨作用的影响

DOI: doi:10.7507/1002-1892.20150048

Keywords: BMSCs, 富白细胞和血小板血浆, 股骨头缺血性坏死, 成骨分化,

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Abstract:

目的探讨富白细胞和血小板血浆(leucocyte- and platelet-rich plasma,L-PRP)在治疗早期股骨头缺血性坏死(avascular necrosis of femoral head,ANFH)兔模型中,对BMSCs成骨分化作用的影响。 方法取4~6月龄新西兰大白兔24只,体重2.0~3.0 kg,雌雄不拘。随机分成A、B、C、D 4组(n=6),建立双侧ANFH模型。取髂骨骨髓分离培养BMSCs并鉴定;采用Landesberg方法制备L-PRP。ANFH动物模型修复方法:A组单纯行髓芯减压,B组行髓芯减压联合L-PRP移植,C组行髓芯减压联合BMSCs移植,D组行髓芯减压联合BMSCs及L-PRP移植。术后2、4、8周摄X线片,观察髋关节和骨缺损灶骨密度变化,并行Lane-Sandhu X线评分评价成骨情况;取各组股骨头标本,行组织学观察及血管计数、新生骨面积百分比检测。 结果影像学及组织学观察均显示各组骨缺损呈现不同程度骨再生。术后2、4、8周,Lane-Sandhu X线片评分、血管计数、新生骨面积百分比均显示:C、D组明显优于A、B组,D组优于C组,B组优于A组,差异均有统计学意义(P<0.05)。 结论L-PRP在治疗兔ANFH模型中对BMSCs成骨分化有促进作用,为临床髓芯减压联合BMSCs及L-PRP治疗ANFH奠定了理论基础

References

[1]  3. 曹亚伟, 王义生, 韩枫, 等. 骨髓间充质干细胞和伞状支撑骨移植术治疗股骨头坏死的实验研究. 中华显微外科杂志, 2010, 33(4):293-296.
[2]  4. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284(5411):143-147.
[3]  7. Dallari D, Fini M, Stagni C, et al. In vitro study on the healing of bone defects treated with bone marrow stromal cells, platelet-rich plasma, and freeze-dried bone allografts, alone and in combination. J Orthop Res, 2006, 24(5):877-888.
[4]  1. 徐俊, 张长青, 孙建琪, 等. 计算机辅助下低温手术建立三足犬股骨头坏死模型的初步报告. 中国修复重建外科杂志, 2008, 22(3):276-280.
[5]  9. Ogino Y, Ayukawa Y, Tsukiyama Y, et al. The effect of platelet-rich plasma on the cellular response of rat bone marrow cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005, 100(3):302-307.
[6]  19. Hernigou P, Beaujean F, Lambotte JC. Decrease of mesenchymal stem cell pool in the upper femoral extremity of patients with osteonecrosis related to corticosteroidtherapy. J Bone Joint Surg (Br), 1999, 81(2):349-355.
[7]  20. Wang BL, Sun W, Shi ZC, et al. Decreased Proliferation of Mesenchymal Stem Cells in Corticosteroid-induced Osteonecrosis of Femoral Head. Orthopedics, 2008, 31(5):444.
[8]  2. Babhulkar S. Osteonecrosis of femoral head:treatment by core decompression and vascular pedicle grafting. Indian J Orthop, 2009, 43(1):27-35.
[9]  5. Wang BL, Sun W, Shi ZC, et al. Treatment of nontraumatic osteonecrosis of the femoral head with the implantation of core decompression and concentrated autologous bone marrow containing mononuclear cells. Arch Orthop Trauma Surg, 2010, 130(7):859-865.
[10]  6. 谢雪涛, 施忠民, 张长青. 富血小板血浆的制备与临床应用研究进展. 中华创伤骨科杂志, 2014, 16(4):311-315.
[11]  8. Arpornmaeklong P, Kochel M, Depprich R, et al. Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. Int J Oral Maxillofac Surg, 2004, 33(1):60-70.
[12]  10. Landsberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg, 2000, 58(3):297-300.
[13]  11. 孙伟, 李子荣, 史振才, 等. 纳米晶胶原基骨和骨髓间充质干细胞复合修复兔股骨头坏死缺损的研究. 中国修复重建外科杂志, 2005, 19(9):703-706.
[14]  12. Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North (Am), 1987, 18(2):213-225.
[15]  13. 李子荣. 科学诊断和治疗股骨头坏死. 中国修复重建外科杂, 2005, 19(9):685-686.
[16]  14. Castro FP Jr, Barrack RL. Core decompression and conservative treatment for avascular necrosis of the femoral head:a meta-analysis. Am J Orthop (Belle Mead NJ), 2000, 29(3):187-194.
[17]  15. Buckley PD, Gearen PF, Petty RW. Structural bone grafting for early atraumatic avascular necrosis of the femoral head. J Bone Joint Surg (Am), 1991, 73(9):1357-1364.
[18]  16. Steinberg ME, Larcom PG, Strafford B, et al. Core decompression with bone grafting for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2001, (386):71-78.
[19]  17. Lieberman JR. Core decompression for osteonecrosis of the hip. Clin Orthop Relat Res, 2004, (418):29-33.
[20]  18. Israelite C, Nelson CL, Ziarani CF, et al. Bilateral core decompression for osteonecrosis of the femoral head. Clin Orthop Relat Res, 2005, (441):285-290.
[21]  21. Kanno T, Takahashi T, Tsujisawa T, et al. Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation. J Oral Maxillofac Surg, 2005, 63(3):362-369.
[22]  22. Oryan A, Meimandi Parizi A, Shafiei-Sarvestani Z, et al. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model:radiological,macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank, 2012, 13(4):639-651.
[23]  23. Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-plasma in rabbit cranial defects:a pilot study. J Oral Maxillofac Surg, 2002, 60(10):1176-1181.
[24]  24. Schlegel KA, Donath K, Rupprecht S, et al. De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials, 2004, 25(23):5387-5393.
[25]  25. Chen L, Yang X, Huang G, et al. Platelet-rich Plasma Promotes Healing of Osteoporotic Fractures. Orthopedics, 2013, 36(6):e687-694.
[26]  26. Thorwarth M, Rupprecht S, Falk S, et al. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials, 2005, 26(15):2575-2584.

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