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-  2015 

趋化因子13在膝关节骨关节炎滑膜中的表达研究

DOI: doi:10.7507/1002-1892.20150010

Keywords: 骨关节炎, 趋化因子13, 滑膜组织

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Abstract:

目的通过与人正常膝关节滑膜比较,观察膝关节骨关节炎滑膜组织中趋化因子13(CXCL13)表达情况,探讨其在骨关节炎发生、发展中的作用。 方法取30例行人工全膝关节置换术的原发性骨关节炎患者滑膜组织(骨关节炎组),男11例,女19例;年龄62~76岁,平均66.7岁。以22例无骨关节炎病史、创伤性截肢患者滑膜组织作为正常对照组,男15例,女7例;年龄48~56岁,平均51.3岁。采用NimbleGen真核生物表达谱芯片检测观察两组差异表达基因,采用免疫组织化学染色、Western blot检测及实时定量PCR检测CXCL13蛋白及基因表达。 结果与正常对照组相比,骨关节炎组基因芯片包含的22 885个基因中上调基因451个,下调基因810个,其中CXCL13基因表达也明显下调。免疫组织化学染色、Western blot检测以及实时定量PCR检测显示,骨关节炎组CXCL13蛋白(0.408 0±0.101 8)及基因(0.011 7±0.003 2)表达,均较正常对照组(0.7859±0.0579、1.0414±0.1297)明显降低,差异均有统计学意义(t=15.630,P=0.000;t=43.634,P=0.000)。 结论膝关节骨关节炎滑膜组织中CXCL13的低表达,可能与骨关节炎发生、发展有关

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