Paper Spray Ionization is an atmospheric pressure ionization technique that utilizes an offline electro-osmotic flow to generate ions off a paper medium. This technique can be performed on a Bruker SolariX Fourier transform ion cyclotron resonance mass spectrometer by modifying the existing nanospray source. High-resolution paper spray spectra were obtained for both organic and biological samples to demonstrate the benefit of linking the technique with a high-resolution mass analyzer. Error values in the range 0.23 to 2.14？ppm were obtained for calf lung surfactant extract with broadband mass resolving power (m/ ) above 60,000 utilizing an external calibration standard. 1. Introduction Since the first practical implementation of electrospray ionization (ESI) with mass spectrometry reported by Yamashita and Fenn in 1984, a number of different atmospheric pressure ionization (API) techniques have been developed [3]. Many API techniques require extensive sample preparation as well as costly consumables to obtain mass spectra. One of the most cost effective and widely available media for chemical separation and analysis is paper. It has been shown that an electro-osmotic flow similar to that of static electrospray can be utilized to interface a triangle of wetted paper to a mass spectrometer (MS); this form of analysis is called paper spray (PS) [4–9]. The use of a chromatographic medium such as paper allows for the analysis of complex mixtures since separations will occur as the analyte is being driven along the paper medium [7]. Separating analytes on a paper medium provides the benefits of reduced probability of ion suppression and rapid analysis. The paper medium allows for analysis of complex samples such as biological tissue and dried blood spots which are not suitable for traditional infusion techniques due to their complex matrices [4, 7]. One limitation to PS, like many MS techniques that utilize incomplete chromatographic separations is the detector being utilized to perform the analysis. When dealing with complex samples or unknown samples, there is a need for high-quality data in order to determine what is being analyzed. Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers are powerful tools for analysis of complex mixtures due to their unsurpassed capability to obtain broadband high-resolution mass spectra with high mass accuracy. Utilizing PS with a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer as a detector allows for rapid analysis of both unknowns and/or complex mixtures. In this present work, we
References
[1]
G. Montaudo, M. S. Montaudo, C. Puglisi, and F. Samperi, “Characterization of polymers by matrix-assisted laser desorption ionization—time of flight mass spectrometry. End group determination and molecular weight estimates in poly(ethylene glycols),” Macromolecules, vol. 28, no. 13, pp. 4562–4569, 1995.
[2]
A. T. Jackson, S. E. Slade, K. Thalassinos, and J. H. Scrivens, “End-group characterisation of poly(propylene glycol)s by means of electrospray ionisation-tandem mass spectrometry (ESI-MS/MS),” Analytical and Bioanalytical Chemistry, vol. 392, no. 4, pp. 643–650, 2008.
[3]
M. Yamashita and J. B. Fenn, “Electrospray ion source. Another variation on the free-jet theme,” Journal of Physical Chemistry, vol. 88, no. 20, pp. 4451–4459, 1984.
[4]
H. Wang, N. E. Manicke, Q. Yang et al., “Direct analysis of biological tissue by paper spray mass spectrometry,” Analytical Chemistry, vol. 83, no. 4, pp. 1197–1201, 2011.
[5]
L. Anyin, H. Wang, Z. Ouyang, and R. G. Cooks, “Paper spray ionization of polar analytes using non-polar solvents,” Chemical Communications, vol. 47, no. 10, pp. 2811–2813, 2011.
[6]
J. Liu, H. Wang, N. E. Manicke, J. M. Lin, R. G. Cooks, and Z. Ouyang, “Development, characterization, and application of paper spray ionization,” Analytical Chemistry, vol. 82, no. 6, pp. 2463–2471, 2010.
[7]
H. Wang, J. Liu, R. Graham Cooks, and Z. Ouyang, “Paper spray for direct analysis of complex mixtures using mass spectrometry,” Angewandte Chemie, vol. 49, no. 5, pp. 877–880, 2010.
[8]
R. G. Cooks, N. E. Manicke, A. L. Dill et al., “New ionization methods and miniature mass spectrometers for biomedicine: DESI imaging for cancer diagnostics and paper spray ionization for therapeutic drug monitoring,” Faraday Discussions, vol. 149, pp. 247–267, 2011.
[9]
R. Haddad, H. M. S. Milagre, R. R. Catharino, and M. N. Eberlin, “Easy ambient sonic-spray ionization mass spectrometry combined with thin-layer chromatography,” Analytical Chemistry, vol. 80, no. 8, pp. 2744–2750, 2008.
[10]
A. G. Marshall, C. L. Hendrickson, and G. S. Jackson, “Fourier transform ion cyclotron resonance mass spectrometry: a primer,” Mass Spectrometry Reviews, vol. 17, no. 1, pp. 1–35, 1998.
[11]
R. H. Notter, E. A. Egan, and M. S. Kwong, “Lung surfactant replacement in premature lambs with extracted lipids from bovine lung lavage: effects of dose, dispersion technique, and gestational age,” Pediatric Research, vol. 19, no. 6, pp. 569–577, 1985.
[12]
E. G. Blugh and W. J. Dyer, “A rapid method of total lipid extraction and purification,” Canadian Journal of Biochemistry and Physiology, vol. 37, no. 8, pp. 911–917, 1959.
