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-  2015 

利用一级神经元重建完全性脊髓损伤大鼠后肢运动功能的实验研究

DOI: doi:10.7507/1002-1892.20150327

Keywords: 脊髓损伤, 一级神经元, 皮质脊髓束, 神经移植, 大鼠

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Abstract:

目的探讨利用一级神经元重建完全性脊髓损伤大鼠后肢运动功能的效果。 方法取40只成年雌性SD大鼠,体质量300~350 g,制备L1椎体水平横断型完全性脊髓损伤模型;模型制备后2周,将大鼠随机分为对照组和实验组(n=20);其中实验组行右后肢一级神经元直接重建手术,对照组除不缝合胫神经远端与股神经近端外,其余处理同实验组。术后观察大鼠一般情况,并于7、30、50、70 d对双侧后肢行BBB评分,观察后肢运动功能恢复情况;于70 d取材行HE染色、神经丝蛋白200(neurofilament 200,NF-200)免疫组织化学染色及辣根过氧化物酶(horseradish peroxidase,HRP)示踪标记染色观察脊髓重建区变化。 结果饲养期间6只大鼠死亡。两组右后肢运动功能无明显恢复,各时间点BBB评分均为0分;左后肢运动功能均有不同程度恢复,但各时间点两组BBB评分比较,差异均无统计学意义(P>0.05)。实验组HE染色可见脊髓重建部位坐骨神经嵌入脊髓中,坐骨神经束膜显示清楚,连接部位脊髓无明显萎缩;NF-200免疫组织化学染色可见脊髓重建部位周围神经内神经元轴突呈阳性染色,且周围神经与脊髓形成联系。HRP逆行示踪标记染色示,实验组脊髓重建部位头端见HRP显色,对照组无着色。 结论一级神经元直接重建手术可使大鼠周围神经与中枢神经通过残留神经元轴突残端再生,重建相应的神经通路,但未见到周围神经支配靶肌肉运动功能的恢复

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