新疆三种葡萄病毒RT-PCR检测及序列分析
RT-PCR detection and sequence analysis on three grapevine viruses from Xinjiang Uyghur Autonomous Region

为研究新疆葡萄中沙地葡萄茎痘伴随病毒(Grapevine rupestris stem pitting associated virus,GRSPaV)、葡萄斑点病毒(Grapevine fleck virus,GFkV)及葡萄病毒A(Grapevine virus A,GVA)的发生情况和新疆分离株系统进化关系,分别克隆3种病毒新疆分离株部分基因区域,应用RT-PCR对新疆64份葡萄样品中上述3种病毒进行检测,并进行系统进化分析.结果显示,GRSPaV、GFkV和GVA的检出率分别为31.3%、62.5%和25.0%.新疆GRSPaV分离株(KJ801847)与美国GRSPaV分离株(AY368590)同源性达96.59%;新疆GFkV分离株(KJ801846)与日本GFkV分离株(AB222861)及中国辽宁GFkV分离株(JF927942)的同源性分别为91.70%和91.03%;新疆GVA分离株(KJ801845)与波兰GVA分离株(JN860997)同源性为93.88%,与中国四川GVA分离株(HQ671655)及辽宁GVA分离株(FJ445220)的同源性分别为92.92%和89.53%.表明3种葡萄病毒在新疆发生比较普遍,且新疆分离株与国内其它地方的分离株存在较大差异.
To detect the grape virus, including Grapevine rupestris stem pitting associated virus (GRSPaV), Grapevine fleck virus (GFkV), Grapevine virus A (GVA), and evaluate their phylogenetic relationships with isolates from other geographic origins, the three kinds of virus were detected by RT-PCR in 64 grape samples from Xinjiang and sequenced. The results showed that the positive percentage with GRSPaV, GFkV and GVA were 31.3%, 62.5% and 25.0%, respectively. The GRSPaV isolate (KJ801847) from Xinjiang shared 96.59% identity with USA isolate (AY368590), and GFkV isolate (KJ801846) shared sequence similarity of 91.70% and 91.03% with Japan isolate (AB222861) and Liaoning (China) isolate (JF927942), respectively. GVA isolate (KJ801845) shared 93.88%, 92.92% and 89.53% identity with Poland isolate (JN860997), Sichuan (China) isolate (HQ671655) and Liaoning (China) isolate (FJ445220), respectively. The detection demonstrated that the three grapevine viruses were widespread distributed in Xinjiang with a significant difference from other isolates in China.