普通小麦-华山新麦草易位系9020-17-25-6抗条锈性遗传分析及分子标记
Genetic and molecular mapping of stripe rust resistance gene in wheat-Psathyrostachys huashanica translocation line 9020-17-25-6

为明确普通小麦-华山新麦草易位系9020-17-25-6的抗条锈病基因及其遗传特点,利用中国条锈菌小种CYR29对9020-17-25-6、铭贤169及其杂交后代F1、F2、F3代进行苗期抗条锈性鉴定及遗传分析,选取48条RGAP引物和491对SSR引物对接种CYR29的F2代群体进行筛选,寻找与抗病基因连锁的分子标记.结果表明:9020-17-25-6对CYR29具有良好的抗条锈性,由1对显性基因独立控制,暂定名为YrHua9020.筛选到2个RGAP标记(M1和M2)和位于染色体3AS上的4个SSR标记(Xwmc11、Xwmc532、Xcfd79、Xgwm2)与YrHua9020连锁,与目的基因的遗传距离分别为6.9、9.5、17.8、12.2、7.2和17.8 cM.与已定位于3A 染色体上的抗条锈病基因的比较研究表明,YrHua9020 是一个与已知基因不同的新的抗条锈病基因.
9020-17-25-6 is a translocation line derived from Triticum aestivum and Psathyrostachys huashanica through cross and back-cross. To study and locate the resistance genes to stripe rust in 9020-17-25-6, the seedlings of F1, F2 and F3 derived from 9020-17-25-6 crossing with susceptible parent Mingxian 169 were tested in greenhouses for genetic analysis of stripe rust resistance. The resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to screen molecular marker linked to the gene conferring resistance to Puccinia striiformis f. sp. tritici race CYR29. The genetic results indicated that 9020-17-25-6 had a single dominant gene conferring resistance to CYR29, temporarily designated as YrHua9020. Two RGAP markers M1 and M2, and four SSR markers Xwmc11, Xwmc532, Xcfd79 and Xgwm2 were tightly linked to the resistance gene on wheat chromosome 3AS, with genetic distance of 6.9, 9.5, 17.8, 12.2, 7.2 and 17.8 cM, respectively. Furthermore, based on chromosomal location, reactions to different pathotypes and pedigree analysis, YrHua9020 was a new resistance gene to stripe rust.