黑龙江省部分地区稻瘟病菌致病性分析及鉴别体系优化
Pathogenicity of Magnaporthe grisea and optimization of differential system in some regions of Heilongjiang

为明确哈尔滨市及鸡西市稻瘟病菌致病性变化情况并优化鉴别体系，以24个水稻单基因系为鉴别体系，对2006-2008年采自2市的稻瘟病菌菌株进行致病型划分，并应用聚类分析方法完成鉴别体系优化。结果表明，应用水稻单基因系鉴别体系可将哈尔滨市2006-2008年采集的稻瘟病菌菌株划分为34、12和27个致病型，致病性相似系数分别为0.32~1.00、0~1.00和0.20~1.00；水稻单基因系对其抗性频率分别为2.86%~97.14%、8.33%~100.00%和10.00%~96.67%；鸡西市2006-2008年采集的菌株可划分为26、19和20个致病型，致病性相似系数分别为0.47~0.92、0.15~0.86和0.39~1.00；水稻单基因系对其抗性频率分别为3.85%~96.15%、5.26%~73.68%和4.76%~95.24%。适用于哈尔滨市的优化鉴别体系包括Pi-9、Pi-11、Pi-a、Pi-ks、Pi-5、Pi-i、Pi-sh、Pi-3、Pi-km和Pi-ta共10个基因，2006-2008年累计方差贡献率分别为86.15%、98.77%和87.39%；适用于鸡西市的优化鉴别体系包括Pi-9、Pi-11、Pi-a、Pi-ks、Pi-5、Pi-i、Pi-sh、Pi-1、Pi-7和Pi-t共10个基因，2006-2008年累计方差贡献率分别为82.58%、94.55%和90.37%。应用优化后的鉴别体系可将哈尔滨市2006-2008年采集的菌株划分为22、12和23个致病型，将鸡西市2006-2008年采集的菌株划分为22、14和18个致病型。
In order to determine the pathogenicity of Magnaporthe grisea from Harbin and Jixi cities and simplify the differential system, 24 monogenic rice lines were used as differential system to divide the pathogenic types and cluster analyses. The results showed that, by using the monogenic rice lines system, M.grisea from Harbin City could be divided into 34, 12 and 27 pathogenic types during 2006-2008, with a similarity coefficient of 0.32-1.00, 0-1.00 and 0.20-1.00, respectively, and the resistance frequency of the monogenic rice lines to M.grisea from Harbin City were 2.86%-97.14%, 8.33%-100.00% and 10.00%-96.67%, respectively. M.grisea from Jixi City could be divided into 26, 19 and 20 pathogenic types during 2006-2008, with a similarity coefficient of 0.47-0.92, 0.15-0.86 and 0.39-1.00, respectively, and the resistance frequency of the monogenic rice lines to M.grisea from Jixi City were 3.85%-96.15%, 5.26%-73.68% and 4.76%-95.24%, respectively. After simplification, the differential system of Harbin City contained Pi-9, Pi-11, Pi-a, Pi-ks, Pi-5, Pi-i, Pi-sh, Pi-3, Pi-km and Pi-ta, whose cumulative variance contribution rates were 86.15%, 98.77% and 87.39% during 2006-2008, respectively. After simplification, the differential system of Jixi City contained Pi-9, Pi-11, Pi-a, Pi-ks, Pi-5, Pi-i, Pi-sh, Pi-1, Pi-7 and Pi-t, whose cumulative variance contribution rates were 82.58%, 94.55% and 90.37% during 2006-2008. By using the differential system of simplification, M.grisea from Harbin City during 2006-2008 was divided into 22, 12 and 23 pathogenic types, while M.grisea from Jixi City during 2006-2008 was divided into 22, 14 and 18 pathogenic types respectively.