CO2气调胁迫对药材甲能源物质的影响
The effect of CO2-enriched atmosphere exposure on the energy sources in drugstore beetle Stegobium paniceum

为明确CO2气调胁迫对药材甲能源物质的影响,采用不同CO2浓度(10%、30%、50%、70%和90%)对其分别胁迫2、4、6、8 h后,测定其体内多糖、可溶性蛋白质及脂肪等能源物质的含量,并计算其利用率,探讨药材甲对气调胁迫的生理生化响应。结果表明,相较于对照,随胁迫时间的延长及CO2浓度的升高,药材甲能源物质的含量均逐渐减少,利用率则逐渐增加。10% CO2浓度胁迫2 h下,单头药材甲多糖、蛋白质及脂肪的含量均最高,分别为13.94、162.87和35.14 μg;其利用率最低,分别为4.85%、2.04%和11.02%。90% CO2浓度胁迫8 h下,其含量最低,分别为11.02、145.69和19.08 μg;利用率最高,分别为24.80%、12.37%和51.68%。不同处理下,药材甲对3种能源物质的利用率均差异显著,为脂肪>多糖>可溶性蛋白质。表明CO2浓度和胁迫时间是导致药材甲能源物质消耗的重要因素,而其对不同能源物质不同的利用率,则可能与昆虫抗气性的形成密切相关。
In order to clarify the effect of CO2-enriched atmosphere exposure on the consumption of energy sources in drugstore beetle Stegobium paniceum, the content of polysaccharides, soluble protein and lipid in S. paniceum were determined, after they were exposed to different CO2-enriched atmospheres (10%, 30%, 50%, 70% and 90%) for 2, 4, 6, and 8 h. Their utilization rates were also calculated to explore the response of insects to CO2 exposure from physiological and biochemical levels. The results showed that the contents of polysaccharides, soluble protein and lipid decreased, but their utilization rates increased, with increasing CO2 concentration under the same exposure time or with the extension of exposure time under the same CO2 concentration, compared to the controls. The highest content and lowest utilization rate of energy source were in the treatment of 2 h exposure under 10% CO2, and the content of polysaccharides, soluble protein and lipid were 13.94, 162.87 and 35.14 μg/insect, with their utilization rates of 4.85%, 2.04% and 11.02%, respectively; the lowest content and highest utilization rate of energy source were in the treatment of 8 h exposure under 90% CO2, with the contents of 11.02, 145.69, 19.08 μg/insect and utilization rates of 24.80%, 12.37% and 51.68%, respectively. In addition, there was significant difference in the utilization rates of the three kinds of energy sources, and the order was lipid > polysaccharides > soluble protein under all the CO2 exposure treatments. Therefore, the CO2 concentration and exposure time were important factors causing energy sources consumption of S. paniceum, and the significantly different utilization rates of different energy sources in insects under different CO2 exposure treatments probably had some associations with their resistance development to CO2.