禾谷镰刀菌CYP51A基因对五种三唑类杀菌剂敏感性的影响
Effect of CYP51A gene of Fusarium graminearum on sensitivity to five triazole fungicides

为进一步明确14α-脱甲基酶编码基因CYP51A在禾谷镰刀菌Fusarium graminearum对三唑类杀菌剂敏感性中的作用，利用Split-marker方法敲除禾谷镰刀菌CYP51A基因并获得敲除CYP51A基因的禾谷镰刀菌ΔFgCYP51A菌株，比较该菌株和野生型菌株PH-1对5种三唑类杀菌剂的敏感性变化。结果表明，ΔFgCYP51A菌株对丙环唑、戊唑醇、烯唑醇、三唑醇和三唑酮的EC50分别为0.024、0.047、0.148、0.154和0.474 mg/L。ΔFgCYP51A菌株对戊唑醇、三唑醇、三唑酮和丙环唑的敏感性均显著增加，而对烯唑醇的敏感性明显变化。表明FgCYP51A基因与禾谷镰刀菌对三唑类杀菌剂的敏感性有关。
To understand the function of 14 alpha-demethylation encoding gene CYP51A on the sensitivity to triazole fungicides, the Split-marker approach was used to generate the FgCYP51A gene deletion mutant, which was named as the ΔFgCYP51A strain, and the sensitivity was investigated and compared between ΔFgCYP51A strain and wide type strain PH-1. The results showed that the EC50 of ΔFgCYP51A strain to propiconazole, tebuconazole, diniconazole, triadimenol and triadimefon were 0.024, 0.047, 0.148, 0.154 and 0.474 mg/L, respectively. The sensitivity of ΔFgCYP51A strain to tebuconazole, triadimenol, triadimefon and propiconazole increased significantly compared to the PH-1 strain, but the diniconazole had no significant change. This research proved that the FgCYP51A gene was sensitive to triazole fungicide.