Q型烟粉虱成虫传播番茄褪绿病毒的能力
Transmission of Tomato chlorosis virus (ToCV) by Bemisia tabaci biotype Q adults

为明确Q型烟粉虱Bemisia tabaci成虫对番茄褪绿病毒（Tomato chlorosis virus，ToCV）的传毒特征，应用RT-PCR技术检测了ToCV在Q型烟粉虱成虫体内的存留时间、Q型烟粉虱成虫对ToCV的获毒效率及传毒效率。结果表明，Q型烟粉虱成虫离开带毒番茄植株后，其带毒率随时间的延长逐渐降低，最初为85.73%，96 h后仅为17.14%。在相同温度下，Q型烟粉虱成虫的获毒率先随着取食时间的延长而提高，而后处于相对稳定状态，当温度为21、26、31℃时，Q型烟粉虱成虫获毒率达到峰值的时间分别为72、48、6 h，最高获毒率分别为56.52%、54.17%和61.64%。当温度为26℃时，Q型烟粉虱成虫的传毒效率随着取食时间的延长而提高，取食时间为0.5 h时，番茄植株带毒率为25.00%，取食时间为48 h时，番茄植株带毒率达91.67%；温度对Q型烟粉虱成虫的传毒效率有极显著影响，取食48 h后，在21~31℃条件下番茄植株带毒率为83.33%~91.67%，在16℃条件下仅为16.67%。表明Q型烟粉虱成虫对ToCV具有较强的获毒和传毒能力，对其进行有效控制可以防止ToCV的扩散和蔓延。
In order to clarify the transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci biotype Q adults, the residence time of ToCV in B. tabaci biotype Q adults, the virus acquisition efficiency and transmission efficiency of B. tabaci biotype Q adults were studied with RT-PCR detection. The rate of B. tabaci biotype Q adult carrying virus reduced gradually with the time after they left the viruliferous tomato plant, which was 85.73% at first and 17.14% after 96 h. At the same temperature, the rate of B. tabaci biotype Q adult acquiring virus increased with the feeding time first and then inclined to be steady. When the experiment temperature were 21, 26 and 31℃, the times that the virus acquisition rate of B. tabaci biotype Q adults reached the peak were 72 h, 48 h and 6 h, respectively, and the highest virus acquisition rate were 56.52%, 54.17% and 61.64%, respectively. At 26℃ , the virus transmission efficiency of B. tabaci biotype Q adult increased with the feeding time. The viruliferous percent of tomato plant was 25.00% when the feeding time was 0.5 h, and it was 91.67% when the feeding time was 48 h. Temperature had significant influence on the virus transmission efficiency of B. tabaci biotype Q adult; when the feeding time was 48 h, the viruliferous percent of tomato plant was 83.33%-91.67% when the temperature was 21-31℃, but it was only 16.67% when the temperature was 16℃. Therefore, B. tabaci biotype Q adult had stronger acquisition efficiency and transmission efficiency for ToCV and should be effectively controlled to prevent the diffusion and spread of ToCV disease.