基于线粒体COI基因序列的大豆食心虫不同地理种群遗传分化
Genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera: Olethreutidae) based on mitochondrial COI gene sequences

为揭示大豆食心虫Leguminivora glycinivorella不同地理种群间的遗传分化情况，通过测定19个地理种群214头老熟幼虫的线粒体COI基因序列，利用MEGA 6.0和DnaSP 5.0软件对不同地理种群间序列变异和遗传分化进行分析。结果表明，在214条408 bp的COI基因序列中发现46个变异位点，获得49种单倍型。总群体单倍型多样度为0.8277，固定系数为0.59，基因流为0.17。总群体Tajima's D检验结果不显著，说明在较近时间内大豆食心虫未经历群体扩张。大豆食心虫不同地理种群间基因交流较少，贵阳种群、都安种群与其它地理种群分化明显，整体的遗传多样性和遗传分化程度较高，地理距离是影响不同地理种群间遗传距离的重要因素。
In order to reveal the genetic differentiation among different geographic populations of Leguminivora glycinivorella, the gene sequences of mitochondrial cytochrome coxidase subunit I (COI) from 214 mature larval individuals of 19 geographic populations were analyzed, and the genetic differentiation and sequence variations were analyzed using MEGA 6.0 and DnaSP 5.0. The results showed that, among the 408 bp alignment of 214 COI sequences, 46 variable sites and 49 haplotypes were identified. The diversity index, total fixed coefficient and total gene flow of total haplotypes were 0.8277, 0.59 and 0.17, respectively. No difference in Tajima's D test among different populations indicated that there might be no population expansion in recent history of L. glycinivorella. It revealed that there was less gene flow among different geographic populations of L. glycinivorella. The Guiyang and Du'an populations showed obvious observed genetic differentiation with other populations. The whole genetic diversity and the genetic differentiation were higher. The geographic distance had important influence on the genetic distance of different geographic populations of L. glycinivorella.