抗虫绿豆对绿豆象生长发育及体内几种酶活性的影响
Effects of insect-resistant mung bean on development and activities of several enzymes of Chinese bean weevil Callosobruchus chinensis L.(Coleoptera:Bruchidae)

为明确抗虫绿豆对绿豆象Callosobruchus chinensis L.生长发育的抑制作用，采用室内人工接虫和生化测定的方法，研究了绿豆象幼虫取食抗虫绿豆后其体重、体内消化酶、保护酶和解毒酶活性及成虫羽化率和体重的变化。结果表明，取食抗虫绿豆的绿豆象幼虫体重、雌、雄成虫体重以及成虫羽化率分别是取食感虫绿豆的0.69、0.69、0.71及0.14倍；取食抗虫绿豆8~16 d的绿豆象幼虫中肠总蛋白酶、α-淀粉酶和α-葡萄糖苷酶活性均显著低于对照；保护酶超氧化物歧化酶、过氧化物酶和过氧化氢酶与解毒酶羧酸酯酶和谷胱甘肽转移酶的活性在绿豆象幼虫取食8~12 d时均显著高于对照，但取食14~16 d时，2种解毒酶活性与对照差异不显著。表明抗虫绿豆可抑制绿豆象幼虫中肠消化酶活性，影响其体内保护酶和解毒酶活性，从而使绿豆象幼虫不能正常生长发育。
To clarify the inhibitory effect of insect-resistant mung bean on growth and development of Chinese bean weevil Callosobruchus chinensis L.,the body weight and activities of enzymes for digestion,protection,and detoxification of larvae treated with insect-resistant mung bean,and the eclosion rate and weight of imagoes were detected after artificial inoculation in the laboratory.The results showed that the larval weight,the adult eclosion rate,and the body weight of the male and female adults fed on insect-resistant mung bean were 0.69,0.14,0.69 and 0.71 times as those on the control,respectively.The activities of total protease,α-amylase,and α-glucosidase in midgut of larvae were significantly decreased after treatment with insect-resistant mung bean (on day 8 and 12) with a concomitant increase in the activities of superoxide dismutase (SOD),peroxidase (POD),carboxylesterase (CarE),and GSH-S-transferase (GST).However,on day 14 and 16,there were no significant differences in the activities of CarE and GST.These results indicated that insect-resistant mung bean could disturb the normal development of C.chinensis larvae by suppressing the digestive enzymes and adversely affecting the activities of protective and detoxifying enzymes.