气相色谱-串联质谱法测定韭菜中的三唑酮和腐霉利残留
Determination of triadimefon and procymidone residues in Allium tuberosum by gas chromatography-tandem mass spectrometry

建立了韭菜中三唑酮和腐霉利同时测定的气相色谱-串联质谱(gas chromatographytandem mass spectrometry,GC-MS/MS)分析方法。样品匀浆后用乙腈提取,经盐析和利用石墨化碳黑/氨基混合型固相萃取柱进行净化,经GC-MS/MS检测,外标法定量。结果表明:在0.01~0.5mg/kg添加水平下,三唑酮和腐霉利回收率在84%~98%之间,相对标准偏差在2.0%~7.3%(n=5)之间;三唑酮和腐霉利的定量限分别为0.005和0.001mg/kg,可满足韭菜中三唑酮和腐霉利残留检测的要求。
For the determination of triadimefon and procymidone in Allium tuberosum Rottl. ex Spreng., an analytical method was established by using gas chromatography-tandem mass spectrometry (GCMS/ MS). The homogenate samples were extracted with acetonitrile, and induced using sodium chloride. Then the extracting solution was purification on carbon/NH2 column. Finally, the concentration was analysised by GC-MS/MS using the external standard method. The results showed that the average recoveries of triadimefon and procymidone were 84%-98% when the spiked levels were 0.01, 0.05, 0.1 and 0.5 mg/kg, with relative standard deviations (RSDs) in the range of 2.0%-7.3% (n = 5). The limits of quantitation (LOQ) for triadimefon and procymidone were 0.005 and 0.001 mg/kg, respectively. The method is viable for the detection of triadimefon and procymidone residues in A. tuberosum.