氯菊酯异构体对人类绒毛膜癌JEG-3细胞内分泌的选择性干扰
Endocrine disruption effect of permethrin isomers to the chorion carcinoma JEG-3 cells

以人类绒毛膜癌JEG-3细胞为模型，通过考察拟除虫菊酯类农药氯菊酯（permethrin，PM）及其异构体对JEG-3细胞内分泌相关基因的干扰情况，探讨了氯菊酯及其异构体暴露对产妇胎儿健康的潜在风险。通过高效液相色谱拆分得到氯菊酯的4个异构体，采用实时荧光定量反转录聚合酶链式反应（reverse transcription polymerase chain reaction，RT-PCR）法检测目的基因的相对表达水平，发现氯菊酯异构体对JEG-3细胞促性腺激素释放激素（GnRHI，GnRHⅡ）及其受体（GnRHR）、胆甾醇类雌激素合成关键基因以及胚胎免疫耐受相关基因（HLA-G）在mRNA水平的相对表达量均呈现选择性干扰，其中1R-cis-PM和1S-trans-PM对滋养层细胞内分泌相关基因表达量的影响大于其他2个异构体。
The endocrine disruption effect of four permethrin isomers to the chorion carcinoma JEG-3 cells was investigated. JEG-3 cell lines was employed as the in vitro model and the potential risks of these compounds to maternal-fetal health were also evaluated. Four permethrin isomers were separated by HPLC. The results of real-time reverse transcription polymerase chain reaction (RT-PCR) revealed that those four isomers, in RNA levels, exhibited selective influence on the relative expression amounts of the genes correspounding to gonadotropin-releasing hormone (GnRH) and its receptor, the enzymes of the steroidogenesis pathway and human leucocyte antigen G (HLA-G). The endocrine disruption effects induced by 1R-cis-PM and 1S-trans-PM were more significant than that induced by 1S-cis-PM and 1R-trans-PM.