九种农药制剂和一种叶面肥中有机磷和氨基甲酸酯类隐性成分的快速检测
Fast identification method of organophosphorus and carbamate in 9 kinds of pesticide formulations and 1 kind of leaf fertillizer

建立了一种针对9种农药制剂和1种叶面肥中隐性添加23种有机磷或氨基甲酸酯类农药的快速检测方法。将农药制剂和叶面肥用磷酸盐缓冲溶液稀释静置后取上清液,加入乙酰胆碱酯酶溶液和显色剂,摇匀后反应5 min,加入底物后通过农药残留速测仪在波长412 nm处测定0~3 min的吸光度变化值。此外,对氧乐果、甲拌磷和甲基对硫磷在9种农药制剂和1种叶面肥中的检出限进行了验证。结果表明:该方法检出限在0.03~4.4 mg/L之间,当在9种农药制剂和1种叶面肥中的添加水平略低于检出限时,其抑制率均<50%(为0~26%),当添加水平接近或略高于检出限时,其抑制率均>50%(为56%~100%)。本方法可用于定性判断农药制剂中是否添加了上述隐性成分。
A simple and rapid method was developed to detect 23 kinds of organophosphorus or carbamates pesticides which were illegally added in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer. The pesticide formulations or leaf fertilizer were diluted with phosphate buffered saline solution and the supernatant was transferred into another test tube where the cholinesterase and chromogenic reagent were added orderly. This mixture was stirred and reacted for 5 min. The final detection was performed by a pesticide residue rapid measuring instrument which is used to measure the absorbance change of the mixture during 0-3 min at the wavelength of 412 nm after the substrate was added. Additionally, the limits of detection of omethoate, phorate and methyl parathion in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizers were verified. It was concluded that the limits of determination were ranged from 0.03 mg/L to 4.4 mg/L. The inhibition rates are <50%(0-26%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are marginally lower than the limit of detection. And they are >50%(56%-100%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are close to or marginally higher than the limit of detection. In conclusion, the hidden compositions mentioned above which are illegally added in pesticide formulations or leaf fertilizer could be qualitatively determined by the present method.