种植转Bt基因棉花不同年限土壤PLFA指纹特征与差异
Effects of consecutive cultivation of Bt transgenic cotton on soil microbial community structure based on the characterization of phospholipids fatty acids

为研究连续种植转Bt基因棉花对土壤微生物群落结构的影响,从连续种植3年转基因棉花晋棉26和中棉所41大田,采集土壤样品,测定磷脂脂肪酸(Phospholipid fatty acid,PLFA).结果表明,不同品种和种植年限对不同生育期土壤微生物影响不同,随着种植年限和棉花残茬进入土壤生态系统,转基因棉花土壤细菌占总量的百分数有降低趋势,革兰氏阳性和阴性细菌的比例低于非转基因品种;转基因棉花土壤真菌的比例逐渐增加.说明转基因棉花连续种植可能改变土壤微生物群落结构.
The experiments were conducted to evaluate the effects of 3 years consecutive cultivation of Bt transgenic cotton on soil microbial community in field at two sites based on phospholipid fatty acid (PLFA) profiles.Two transgenic Bt varieties (JM26,CCRI41) and one non-Bt cotton variety (JM7) were used in Yanhu district,Yuncheng site,JM26 and JM7 in Xiaxian county site .The results showed that the total microbial PLFA content were different in different stages,variety and years.The bacteria rate and ratio of Gram-positive and Gram-negative in transgenic cotton were lower than that in non-Bt cotton with consecutive cultivation.The microbial community was characterized by the predominance of fungi in the Bt cotton soil.The soil microbial community had been changed in the Bt cotton soil with consecutive cultivation compared with non-Bt cotton soil.