转LTP基因T0代小麦的获得及抗条锈病鉴定
Wheat T0 generation of transgenic LTP genes and identification of resistance to stripe rust

以小麦生产品种小偃22、科农199和西农1376为受体品种,植物抗菌蛋白基因LTP作为目的基因,构建pAHC25-LTP表达载体,利用基因枪法转化小麦幼胚愈伤组织,获得转LTP基因T0代小麦1247株.经草铵膦(Phosphinothricin,PPT)抗性筛选及PCR分子检测,获得阳性再生植株209株,转化率1.87%.阳性再生植株接种条锈菌CYR29、CYR31和CYR32混合小种后进行抗条锈病鉴定,获得抗性植株74株,其中抗性显著提高的抗病植株11株.本研究初步证明抗菌蛋白基因LTP在小麦抗条锈病菌的侵染中有一定作用,为获得转抗菌蛋白基因的抗条锈病小麦品种奠定基础,以期获得育种中可应用的新型抗源材料.
In order to transform the plant antibacterial protein gene LTP into the XiaoYan 22,KeNong 199 and XiNong 1376 clutivars,expression vector pAHC25-LTP was constructed and transformed into immature embryo callus by biolistic bombardment,thereby generating 1247 T0 plants.Among them,209 plants were validated,by phosphinothricin (PPT) screening and PCR detection,to be positive transgenic plants.The transformation rate was 1.87%.Resistance tests,with the mixed infection of Pst (Puccinia striiformis f.sp.Tritici) CYR29,CYR31 and CYR32,showed that 74 plants had resistance and 11 plants resistance levels increased significantly.These results preliminarily illustrate that LTP plays a role in wheat resistance response against stripe rust infection.This study lays a foundation to obtain antibacterial protein transgenic varieties and new resistance materials for wheat breeding.