短柄草Hsf家族全基因组鉴定、分类和高温响应
Genome-wide identification,classification and high temperature responses of Hsf family in Brachypodium distachyon

分析短柄草Hsf家族成员的进化关系,并进行分类;利用多种软件和在线工具分析短柄草Hsf的蛋白结构功能域和基因启动子区域的顺式作用元件;分析基因组信息,进行基因的染色体定位;利用荧光实时定量技术(qRT-PCR),分析短柄草Hsf基因在高温胁迫下的表达模式,鉴定出一些高温诱导表达的Hsf基因。
High temperature stress is one of the most important abiotic stress factors that affect the crop yield and quality.Heat shock response is mainly regulated by heat shock transcription factor (Hsf).Softwares and online tools were used to analyze the Hsf gene structures,protein function domains,chromosomal locations,phylogenetic relationships and cis-elements.Twenty-four Hsf genes were systematically identified,classified into 13 subclasses according to their phylogenetic relationships and located on 5 chromosomes.Each of the Hsf genes contained introns and each contained cis-elements in their promoter regions which might participate in drought,low temperature,high temperature,high light and ABA stress responses.Quantitative real-time PCR (qRT-PCR) was used to analyze the expressions of the Hsf genes under high temperature stress and most of the Hsf genes showed up-regulated.In this work,identification,chromosomal location,classification,genetic structure,protein structure and expression analysis of Brachypodium distachyon Hsf genes under high temperature condition would provide the theory basis for the further research on the functions of Hsf genes.