油用向日葵GDSL脂酶/脂肪酶基因的序列分析
Sequence analysis of GDSL esterase/lipase genes in Helianthus annuus L.

为挖掘油用向日葵中的GDSL脂肪酶基因，本研究以油用向日葵转录组测序得到的Unigenes为对象，筛选GDSL脂酶/脂肪酶基因并对其理化性质和序列结构等特点进行生物信息学分析。结果表明：油用向日葵中筛选到7个GDSL脂酶/脂肪酶基因，编码的蛋白质为稳定存在的亲水性蛋白，由255~396个氨基酸组成，分子量介于28.75~42.61 ku，等电点分布在4.91~8.89；除unigene 60916外，编码的蛋白质都含有较多的跨膜区域和较高的磷酸化程度；α-螺旋和无规则卷曲是主要的二级结构；进化树分析表明，这7个基因聚为三类，保守性较高，其中unigene 58624与AT4G01130.1在同一分支上高度保守，unigene 60916与AT1G74460.1相似度最高，含有多个α螺旋和β折叠，推测与种子的油脂代谢有关。
In order to exploit GDSL esterase/lipase gene of Helianthus annuus L, GDSL esterase/lipase genes were screened based on the Unigenes obtained from H. annuus's transcriptome sequencing data in this study. The selected GDSL esterase/lipase gene and analysis on its physicochemical properties and sequence structure was conducted by Bioinformation. The results showed that:Seven GDSL esterase/lipase genes were selected from H. annuus, with the molecular size ranged from 255 to 396 aa. The ranges of molecular weight and isoelectric point were respectively 28.75~42.61 ku and 4.91~8.89. Besides unigene60916, most proteins had more transmembrane regions and higher phosphorylation levels; The alpha helix and the irregular curl were the primary secondary structure. The constructed phylogenetic tree indicated that the 7 genes were well grouped into three categories. The 7 genes are more conservative. Among which, unigene 58624 was highly conservative with AT4G01130.1 on the same branch. Unigene 60916was speculated to be related to lipid metabolism in seeds, which had the highest similarity with AT1G74460.1 and contained multiple alpha helix and beta sheet.