玉米ZmPGP1基因启动子的克隆及结构功能分析
Cloning and functional analysis of maize ZmPGP1 promoter

为分析玉米ZmPGP1基因启动子的功能，利用巢式PCR方法克隆出了玉米ZmPGP1基因的启动子调控区，并将该启动子与GUS基因融合，通过基因枪法转入玉米（Zea mays）中，分析ZmPGP1启动子表达特性。结果显示，在玉米中克隆出ZmPGP1基因5'端上游1 090 bp的启动子序列，该启动子序列包括光响应元件、激素响应元件和胁迫诱导及发育相关顺式作用元件。GUS染色表明ZmPGP1基因在玉米幼苗的茎部、叶子及根中都有表达，其中茎的节间处以及叶鞘部位表达量较高，这与ZmPGP1基因的Real-time PCR分析结果一致。研究结果进一步阐明ZmPGP1基因的功能以及作用机理。
To analyze the function of maize ZmPGP1 gene promoter,the sequence of ZmPGP1 promoter was cloned by nest-PCR.The cloned promoter was fused with GUS gene and then introduced into maize by particle bombardment to investigate the expression feature of ZmPGP1 gene.The results showed that the length of cloned ZmPGP1 promoter was 1 090 bp containing light-,hormone-,stress induced-and development-related cis-elements.The results from histochemical GUS staining revealed that ZmPGP1 expressed in stem,leaf and root,and its expression level was high in internodes and sheath,which was consistent with the expression analysis of ZmPGP1 by Real-time PCR.These results provided references for further function and mechanism study of ZmPGP1 action.